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Ligands
Code Name Style Show Link
Non-standard Residues
Code Name Show
FHU (5s,6r)-5-fluoro-6-hydroxy-pseudouridine-5'-monophosphate
Glycosylation
Code Name Emphasize
Modification
Code Name Show
Code : 1ZE2   PDBj   RCSB PDB   PDBe
Header : LYASE/RNA
Title : Conformational change of pseudouridine 55 synthase upon its association with RNA substrate
Release Data : 2005-04-26
Compound :
mol_id molecule chains
1 5'-R(*GP*GP*CP*CP*AP*CP*GP*GP*UP*(FHU)P*CP*GP*AP*AP*UP*CP*CP*GP*UP*GP*GP*C)-3' C,D
mol_id molecule chains synonym
2 tRNA pseudouridine synthase B A,B tRNA pseudouridine 55 synthase, Psi55 synthase, Pseudouridylate synthase, Uracil hydrolyase
ec: 4.2.1.70
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific expression_system
2 Thermotoga maritima  (taxid:2336) Escherichia coli BL21(DE3)  (taxid:469008)
gene: truB
expression_system_strain: BL21DE3
expression_system_vector_type: Plasmid
expression_system_plasmid: PLM1
Authors : Phannachet, K., Huang, R.H.
Keywords : Protein-RNA complex, LYASE-RNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 3.0 Å )
Citation :

Conformational change of pseudouridine 55 synthase upon its association with RNA substrate

Phannachet, K.,Huang, R.H.
(2004)  Nucleic Acids Res.  32 : 1422 - 1429

PubMed: 14990747
DOI: 10.1093/nar/gkh287

Chain : A, B
UniProt : Q9WZW0 (TRUB_THEMA)
Reaction: EC: Evidence:
Physiological Direction:
uridine(55) in tRNA = pseudouridine(55) in tRNA 5.4.99.25 HAMAP-Rule:MF_01080
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