Brand  (β version)

PDB ID: 1YVZ

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Ligands
Code Name Style Show Link
JPC 3-[(2,4-dichlorobenzoyl)(isopropyl)amino]-5-phenylthiophene-2-carboxylic acid PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1YVZ   PDBj   RCSB PDB   PDBe
Header : VIRAL PROTEIN
Title : Hepatitis C Virus RNA Polymerase Genotype 2a In Complex With Non- Nucleoside Analogue Inhibitor
Release Data : 2005-03-22
Compound :
mol_id molecule chains
1 RNA dependent RNA polymerase A
other_details: genotype 2a
Source :
mol_id organism_scientific expression_system
1 Hepatitis C virus  (taxid:11103) Escherichia coli BL21(DE3)  (taxid:469008)
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET-21b
Authors : Biswal, B.K., Cherney, M.M., Wang, M., Chan, L., Yannopoulos, C.G., Bilimoria, D., Nicolas, O., Bedard, J., James, M.N.G.
Keywords : NS5B POLYMERASE GENOTYPE 2a, NON-NUCLEOSIDE INHIBITOR, Viral protein
Exp. method : X-RAY DIFFRACTION ( 2.20 Å )
Citation :

Crystal structures of the RNA dependent RNA polymerase genotype 2a of hepatitis C virus reveal two conformations and suggest mechanisms of inhibition by non-nucleoside inhibitors.

Biswal, B.K.,Cherney, M.M.,Wang, M.  et al.
(2005)  J.Biol.Chem.  280 : 18202 - 18210

PubMed: 15746101
DOI: 10.1074/jbc.M413410200

Reaction

Chain : A
UniProt : P26660 (POLG_HCVJ6)
Reaction: EC: Evidence:
Physiological Direction:
[Serine protease/helicase NS3]
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'. 		3.4.21.98 UniProtKB:P27958
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[Serine protease/helicase NS3]
a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'- diphosphate + phosphate + H(+) 		3.6.1.15 UniProtKB:P27958
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[Serine protease/helicase NS3]
ATP + H2O = ADP + phosphate + H(+) 		3.6.4.13 UniProtKB:P27958
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[RNA-directed RNA polymerase]
RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate 		2.7.7.48 PROSITE-ProRule:PRU00539, PubMed:21209117
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Cofactor: Evidence: Note:
Zn(2+) ECO:0000250 | UniProtKB:P26663
Activity of protease NS2 is dependent on zinc ions and completely inhibited by EDTA. This is probably due to the fact that NS2 protease activity needs NS3 N-terminus that binds a zinc atom (active region NS2-3).
Mg(2+) ECO:0000250 | UniProtKB:P26663
ECO:0000250 | UniProtKB:Q9WMX2
Binds 1 zinc ion, which has a structural role (By similarity). The magnesium ion is essential for the helicase activity (By similarity).
Mg(2+) ECO:0000250 | UniProtKB:P26663
Binds 2 magnesium ion that constitute a dinuclear catalytic metal center.