Brand  (β version)

PDB ID: 1YQN

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
CDP Cytidine-5'-diphosphate PoSSuM
GPP Geranyl diphosphate PoSSuM
MN Manganese (II) ion PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 1YQN   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : E. coli ispF double mutant
Release Data : 2005-07-12
Compound :
mol_id molecule chains synonym
1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase A Cytidine 5-diphosphate, MECPS, MECDP-synthase
ec: 4.6.1.12
mutation: R142M, E144L
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli BL21(DE3)  (taxid:469008)
gene: ispF
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET15b
Authors : Sgraja, T., Kemp, L.E., Ramsden, N., Hunter, W.N.
Keywords : 2C-methyl-D-erythriol-2, 4-cyclodiphosphate synthase R142M/E144K (ispF mutant), alpha-beta protein, LYASE
Exp. method : X-RAY DIFFRACTION ( 3.11 Å )
Citation :

A double mutation of Escherichia coli2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase disrupts six hydrogen bonds with, yet fails to prevent binding of, an isoprenoid diphosphate.

Sgraja, T.,Kemp, L.E.,Ramsden, N.  et al.
(2005)  Acta Crystallogr.,Sect.F  61 : 625 - 629

PubMed: 16511114
DOI: 10.1107/S1744309105018762

Reaction

Chain : A
UniProt : P62617 (ISPF_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D- erythritol 2,4-cyclic diphosphate + CMP 4.6.1.12 HAMAP-Rule:MF_00107, PubMed:10694574, PubMed:22839733
-
4-CDP-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 3,4- cyclophosphate + CMP + H(+) - PubMed:10694574
-