Brand  (β version)

PDB ID: 1Y60

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Ligands
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H4M 5,10-dimethylene tetrahydromethanopterin PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1Y60   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : Structure of the tetrahydromethanopterin dependent formaldehyde-activating enzyme (Fae) from Methylobacterium extorquens AM1 with bound 5,10-methylene tetrahydromethanopterin
Release Data : 2005-01-11
Compound :
mol_id molecule chains
1 Formaldehyde-activating enzyme fae A,B,C,D,E
other_details: complexed with pterin-related cofactor
Source :
mol_id organism_scientific expression_system
1 Methylobacterium extorquens  (taxid:272630) Escherichia coli  (taxid:562)
strain: AM1
gene: fae
expression_system_strain: BL21(DE3)pLysS
expression_system_vector_type: Plasmid
expression_system_plasmid: pET17b
Authors : Acharya, P., Goenrich, M., Hagemeier, C.H., Demmer, U., Vorholt, J.A., Thauer, R.K., Ermler, U.
Keywords : Pentamer; beta-alpha-beta left handed crossover; TETRAHYDROMETHANOPTERIN-BINDING, LYASE
Exp. method : X-RAY DIFFRACTION ( 1.90 Å )
Citation :

How an enzyme binds the C1-carrier tetrahydromethanopterin: Structure of the tetrahydromethanopterin dependent formaldehyde-activating enzyme (Fae) from Methylobacterium extorquens AM1

Acharya, P.,Goenrich, M.,Hagemeier, C.H.  et al.
(2005)  J.Biol.Chem.  280 : 13712 - 13719

PubMed: 15632161
DOI: 10.1074/jbc.M412320200

Reaction

Chain : A, B, C, D, E
UniProt : Q9FA38 (FAE_METEA)
Reaction: EC: Evidence:
Physiological Direction:
5,6,7,8-tetrahydromethanopterin + formaldehyde = 5,10- methylenetetrahydromethanopterin + H2O 4.2.1.147 PubMed:11073907, PubMed:15632161
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