Brand  (β version)

PDB ID: 1XMZ

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Ligands
Code Name Style Show Link
BME Beta-mercaptoethanol PoSSuM
Non-standard Residues
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CRK 4-{(z)-[2-[3-(methylsulfanyl)propanoyl]-5-oxo-1-(2-oxoethyl)-1,5-dihydro-4h-imidazol-4-ylidene]methyl}benzenolate
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NH2 Amino group

Structure summary

Code : 1XMZ   PDBj   RCSB PDB   PDBe
Header : LUMINESCENT PROTEIN
Title : Crystal structure of the dark state of kindling fluorescent protein kfp from anemonia sulcata
Release Data : 2005-04-19
Compound :
mol_id molecule chains
1 GFP-like non-fluorescent chromoprotein FP595 chain 1 A,B
mol_id molecule chains
2 GFP-like non-fluorescent chromoprotein FP595 chain 2 C,D
mutation: A143G
Source :
mol_id organism_scientific organism_common expression_system
1 Anemonia sulcata  (taxid:6108) Snake-locks sea anemone Escherichia coli  (taxid:562)
expression_system_strain: JM-109
expression_system_vector_type: PLASMID
expression_system_vector: PLASMID
expression_system_plasmid: PQE-30
mol_id organism_scientific organism_common expression_system
2 Anemonia sulcata  (taxid:6108) Snake-locks sea anemone Escherichia coli  (taxid:562)
expression_system_strain: JM-109
expression_system_vector_type: PLASMID
expression_system_vector: PLASMID
expression_system_plasmid: PQE-30
Authors : Quillin, M.L., Anstrom, D.M., Shu, X., O'Leary, S., Kallio, K., Chudakov, D.M., Remington, S.J.
Keywords : FLUORESCENT PROTEIN, CHROMOPHORE STRUCTURE, ASCP595, LUMINESCENT PROTEIN
Exp. method : X-RAY DIFFRACTION ( 1.38 Å )
Citation :

Kindling Fluorescent Protein from Anemonia sulcata: Dark-State Structure at 1.38 Resolution

Quillin, M.L.,Anstrom, D.M.,Shu, X.  et al.
(2005)  Biochemistry  44 : 5774 - 5787

PubMed: 15823036
DOI: 10.1021/bi047644u

Natural Animal Coloration Can be Determined by a Nonfluorescent Green Fluorescent Protein Homolog

Lukyanov, K.A.,Fradkov, A.F.,Gurskaya, N.G.  et al.
(2000)  J.Biol.Chem.  275 : 25879

Alternative Cyclization in Gfp-Like Proteins Family. The Formation and Structure of the Chromophore of a Purple Chromoprotein from Anemonia Sulcata

Martynov, V.I.,Savitsky, A.P.,Martynova, N.Y.  et al.
(2001)  J.Biol.Chem.  276 : 21012

Interconversion of Anthozoa Gfp-Like Fluorescent and Nonfluorescent Proteins by Mutagenesis

Bulina, M.E.,Chudakov, D.M.,Mudrik, N.N.  et al.
(2002)  BMC BIOCHEM.  3

Chromophore Environment Provides Clue to "Kindling Fluorescent Protein" Riddle

Chudakov, D.M.,Foefanov, A.V.,Mudrik, N.N.  et al.
(2002)  J.Biol.Chem.  278 : 7215

Kindling Fluorescent Proteins for Precise in Vivo Photolabeling

Chudakov, D.M.,Belousov, V.V.,Zaraisky, A.G.  et al.
(2003)  NAT.BIOTECHNOL.  21 : 191

Synthesis and Properties of the Chromophore of Asulcp Chromoprotein from Anemonia Sulcata

Yampolsky, I.V.,Remington, S.J.,Potapov, V.K.  et al.
To be Published 

Reaction

Chain : C, D
UniProt : Q9GZ28 (NFCP_ANESU)
Reaction : -