Brand  (β version)

PDB ID: 1XC8

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Ligands
Code Name Style Show Link
GOL Glycerol PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
Code Name Show
FOX ((1r,2s,4r)-4-{[2-amino-5-(formylamino)-6-oxo-3,6-dihydropyrimidin-4-yl]amino}-2-hydroxycyclopentyl)methyl 5'-phosphate
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 1XC8   PDBj   RCSB PDB   PDBe
Header : HYDROLASE/DNA
Title : CRYSTAL STRUCTURE COMPLEX BETWEEN THE WILD-TYPE LACTOCOCCUS LACTIS FPG (MUTM) AND A FAPY-DG CONTAINING DNA
Release Data : 2005-09-06
Compound :
mol_id molecule chains
1 5'-D(*CP*TP*CP*TP*TP*TP*(FOX)P*TP*TP*TP*CP*TP*CP*G)-3' B
mol_id molecule chains
2 5'-D(*GP*CP*GP*AP*GP*AP*AP*AP*CP*AP*AP*AP*GP*A)-3' C
mol_id molecule chains synonym
3 Formamidopyrimidine-DNA glycosylase A FAPY-DNA glycosylase
ec: 3.2.2.23
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific expression_system
3 Lactococcus lactis subsp. cremoris  (taxid:1359) Escherichia coli  (taxid:562)
strain: subsp. cremoris
variant: subsp. cremoris
gene: mutm, fpg
expression_system_vector_type: PLASMID
expression_system_plasmid: PMAL-C
Authors : Coste, F., Ober, M., Carell, T., Boiteux, S., Zelwer, C., Castaing, B.
Keywords : PROTEIN-DNA COMPLEX; GLYCOSYLASE; FAPY, HYDROLASE-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 1.95 Å )
Citation :

Structural basis for the recognition of the FapydG lesion (2,6-diamino-4-hydroxy-5-formamidopyrimidine) by formamidopyrimidine-DNA glycosylase.

Coste, F.,Ober, M.,Carell, T.  et al.
(2004)  J.Biol.Chem.  279 : 44074 - 44083

PubMed: 15249553
DOI: 10.1074/jbc.M405928200

Reaction

Chain : A
UniProt : P42371 (FPG_LACLC)
Reaction: EC: Evidence:
Physiological Direction:
Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N- methyl)formamidopyrimidine. 3.2.2.23 -
-
2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'- deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho- 2'-deoxyribonucleoside-DNA + H(+) 4.2.99.18 -
-