Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
ACT Acetate ion
FLU 2-(6-hydroxy-3-oxo-3h-xanthen-9-yl)-benzoic acid
Code : 1X9Q
Header : IMMUNE SYSTEM
Title : 4m5.3 anti-fluorescein single chain antibody fragment (scFv)
Release Data : 2005-03-22
Compound :
mol_id molecule chains synonym
1 4m5.3 anti-fluorescein single chain antibody fragment A scFv
fragment: antibody binding fragment
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Saccharomyces cerevisiae  (taxid:4932)
expression_system_common: Baker's yeast
expression_system_strain: YVH10
expression_system_vector_type: pRS316
expression_system_plasmid: GAL1-10 promoter
Authors : Midelfort, K.S., Hernandez, H.H., Lippow, S.M., Tidor, B., Drennan, C.L., Wittrup, K.D.
Keywords : very high affinity, antibody binding, electrostatics, directed evolution, single chain antibody, IMMUNE SYSTEM
Exp. method : X-RAY DIFFRACTION ( 1.5 Å )
Citation :

Substantial energetic improvement with minimal structural perturbation in a high affinity mutant antibody

Midelfort, K.S.,Hernandez, H.H.,Lippow, S.M.  et al.
(2004)  J.Mol.Biol.  343 : 685 - 701

PubMed: 15465055
DOI: 10.1016/j.jmb.2004.08.019