Brand  (β version)

PDB ID: 1X9N

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Ligands
Code Name Style Show Link
AMP Adenosine monophosphate PoSSuM
Non-standard Residues
Code Name Show
DOC 2',3'-dideoxycytidine-5'-monophosphate
MSE Selenomethionine
Glycosylation
Code Name Emphasize
Modification
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Structure summary

Code : 1X9N   PDBj   RCSB PDB   PDBe
Header : Ligase/DNA
Title : Crystal Structure of Human DNA Ligase I bound to 5'-adenylated, nicked DNA
Release Data : 2004-11-30
Compound :
mol_id molecule chains
1 dideoxy terminated DNA B
mol_id molecule chains
2 5'-phosphorylated DNA C
mol_id molecule chains
3 template DNA D
mol_id molecule chains synonym
4 DNA ligase I A Polydeoxyribonucleotide synthase [ATP]
ec: 6.5.1.1
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific
3
synthetic: yes
mol_id organism_scientific organism_common expression_system
4 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: LIG1
expression_system_strain: BL21(DE3)RP
expression_system_vector_type: plasmid
expression_system_plasmid: pET24a
Authors : Pascal, J.M., O'Brien, P.J., Tomkinson, A.E., Ellenberger, T.
Keywords : DNA ligase, 5'-adenylated nicked DNA, protein-DNA complex, Ligase-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 3.00 Å )
Citation :

Human DNA ligase I completely encircles and partially unwinds nicked DNA.

Pascal, J.M.,O'Brien, P.J.,Tomkinson, A.E.  et al.
(2004)  Nature  432 : 473 - 478

PubMed: 15565146
DOI: 10.1038/nature03082

Reaction

Chain : A
UniProt : P18858 (DNLI1_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho- (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate. 6.5.1.1 PROSITE- ProRule:PRU10135
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