Brand  (β version)

PDB ID: 1WXI

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Ligands
Code Name Style Show Link
AMP Adenosine monophosphate PoSSuM
DPO Diphosphate PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
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Glycosylation
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Modification
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Structure summary

Code : 1WXI   PDBj   RCSB PDB   PDBe
Header : LIGASE
Title : E.coli NAD Synthetase, AMP.PP
Release Data : 2005-02-15
Compound :
mol_id molecule chains synonym
1 NH(3)-dependent NAD(+) synthetase A NAD Synthetase, Nitrogen-regulatory protein
ec: 6.3.1.5
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli BL21(DE3)  (taxid:469008)
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pRSETHE
Authors : Jauch, R., Humm, A., Huber, R., Wahl, M.C.
Keywords : NADE, NAD, E.coli, Ligase
Exp. method : X-RAY DIFFRACTION ( 1.7 Å )
Citation :

Structures of Escherichia coli NAD Synthetase with Substrates and Products Reveal Mechanistic Rearrangements

Jauch, R.,Humm, A.,Huber, R.  et al.
(2005)  J.Biol.Chem.  280 : 15131 - 15140

PubMed: 15699042
DOI: 10.1074/jbc.M413195200

Reaction

Chain : A
UniProt : P18843 (NADE_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
ATP + deamido-NAD(+) + NH4(+) = AMP + diphosphate + H(+) + NAD(+) 6.3.1.5 HAMAP-Rule:MF_00193, PubMed:8195100
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