Brand  (β version)

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Ligands
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ARS Arsenic
BST 6-(4-amino-4-hydroxy-2-oxo-3,4-dihydro-2h-pyrimidin-1-yl)-3-[3-amino-5-(N-methyl-guanidino)-pent anoylamino]-3,6-dihydro-2h-pyran-2-carboxylic acid
ZN Zinc ion
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Code : 1WN6   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Crystal Structure of Blasticidin S Deaminase (BSD) Complexed with Tetrahedral Intermediate of Blasticidin S
Release Data : 2005-10-25
Compound :
mol_id molecule chains
1 Blasticidin-S deaminase A,B
ec: 3.5.4.23
Source :
mol_id organism_scientific expression_system
1 Aspergillus terreus  (taxid:33178) Escherichia coli  (taxid:562)
strain: S-712
expression_system_vector_type: plasmid
expression_system_plasmid: pET-12a
Authors : Kumasaka, T., Yamamoto, M., Furuichi, M., Nakasako, M., Kimura, M., Yamaguchi, I., Ueki, T.
Keywords : hydrolase, CYTIDINE DEAMINASE FAMILY, ZINC, TETRAMER
Exp. method : X-RAY DIFFRACTION ( 1.80 Å )
Citation :

Crystal structures of blasticidin S deaminase (BSD): implications for dynamic properties of catalytic zinc

Kumasaka, T.,Yamamoto, M.,Furuichi, M.  et al.
(2007)  J.Biol.Chem.  282 : 37103 - 37111

PubMed: 17959604
DOI: 10.1074/jbc.M704476200

Crystallization and preliminary X-ray diffraction studies of blasticidin S deaminase from Aspergillus terreus

Nakasako, M.,Kimura, M.,Yamaguchi, I.
(1999)  Acta Crystallogr.,Sect.D  55 : 547 - 548

PubMed: 10089374
DOI: 10.1107/S0907444998011809

Chain : A, B
UniProt : P78986 (BSD_ASPTE)
Reaction: EC: Evidence:
Physiological Direction:
blasticidin S + H(+) + H2O = deaminohydroxyblasticidin S + NH4(+) 3.5.4.23 -
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