Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
C Cytidine-5'-monophosphate
MG Magnesium ion
EDO 1,2-ethanediol
GPP Geranyl diphosphate
Code : 1W55
Header : TRANSFERASE
Title : Structure of the Bifunctional IspDF from Campylobacter jejuni
Release Data : 2004-10-04
Compound :
mol_id molecule chains synonym
1 ISPD/ISPF BIFUNCTIONAL ENZYME A 2-C-METHYL-D-ERYTHRITOL 4-PHOSPHATE CYTIDYLYLTRANSFERASE, 4-DIPHOSPHOCYTIDYL-2C-METHYL-D-ERYTHRITOL SYNTHASE, MEP CYTIDYLYLTRANSFERASE, MCT, 2-C-METHYL-D-ERYTHRITOL 2,4-CYCLODIPHOSPHATE SYNTHASE, MECPS, MECDP-SYNTHASE, METHYLERYTHRITOL 4-PHOSPHATE CYTIDYLYLTRANSFERASE, METHYLERYTHRITOL 2,4-CYCLODIPHOSPHATE SYNTHASE
ec: 2.7.7.60, 4.6.1.12
Source :
mol_id organism_scientific expression_system
1 CAMPYLOBACTER JEJUNI  (taxid:197) ESCHERICHIA COLI  (taxid:511693)
strain: JEJUNI NTCT 11168
atcc: 33560
expression_system_strain: BL21
expression_system_vector: PET15B
Authors : Gabrielsen, M., Bond, C.S., Hunter, W.N.
Keywords : BIFUNCTIONAL ENZYME, BIOSYNTHETIC PATHWAY, ISOPRENOIDS, BIFUNCTIONAL, NONMEVALONATE, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.30 Å )
Citation :

Hexameric Assembly of the Bifunctional Methylerythritol 2,4-Cyclodiphosphate Synthase and Protein-Protein Associations in the Deoxy-Xylulose-Dependent Pathway of Isoprenoid Precursor Biosynthesis

Gabrielsen, M.,Bond, C.S.,Hallyburton, I.  et al.
(2004)  J.Biol.Chem.  279 : 52753

PubMed: 15466439
DOI: 10.1074/JBC.M408895200

Chain : A
UniProt : Q9PM68 (ISPDF_CAMJE)
Reaction : CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.
2-phospho-4-(cytidine 5'-diphospho)-2-C- methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP.