Brand  (β version)

PDB ID: 1VRQ

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Ligands
Code Name Style Show Link
SO4 Sulfate ion PoSSuM
NAD Nicotinamide-adenine-dinucleotide PoSSuM
FON N-{[4-({[(6r)-2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)phenyl]carbonyl}-L-glutamic acid PoSSuM
FAD Flavin-adenine dinucleotide PoSSuM
FMN Flavin mononucleotide PoSSuM
DMG N,N-dimethylglycine PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1VRQ   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Crystal Structure of Heterotetrameric Sarcosine Oxidase from Corynebacterium sp. U-96 in complex with Folinic Acid
Release Data : 2005-07-05
Compound :
mol_id molecule chains
1 Sarcosine oxidase alpha subunit A
ec: 1.5.3.1
mol_id molecule chains
2 Sarcosine oxidase beta subunit B
ec: 1.5.3.1
mol_id molecule chains
3 Sarcosine oxidase gamma subunit C
ec: 1.5.3.1
fragment: residues 1-200
mol_id molecule chains
4 Sarcosine oxidase delta subunit D
ec: 1.5.3.1
Source :
mol_id organism_scientific expression_system
1 Corynebacterium sp.  (taxid:31944) Escherichia coli BL21(DE3)  (taxid:469008)
strain: U-96
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET31b
mol_id organism_scientific expression_system
2 Corynebacterium sp.  (taxid:31944) Escherichia coli BL21(DE3)  (taxid:469008)
strain: U-96
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET31b
mol_id organism_scientific expression_system
3 Corynebacterium sp.  (taxid:31944) Escherichia coli BL21(DE3)  (taxid:469008)
strain: U-96
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET31b
mol_id organism_scientific expression_system
4 Corynebacterium sp.  (taxid:31944) Escherichia coli BL21(DE3)  (taxid:469008)
strain: U-96
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET31b
Authors : Ida, K., Moriguchi, T., Suzuki, H.
Keywords : Heterotetrameric sarcosine oxidase, flavoenzyme, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 2.20 Å )
Citation :

Crystal structure of heterotetrameric sarcosine oxidase from Corynebacterium sp. U-96

Ida, K.,Moriguchi, T.,Suzuki, H.
(2005)  BIOCHEM.BIOPHYS.RES.COMMUN.  333 : 359 - 366

PubMed: 15946648
DOI: 10.1016/j.bbrc.2005.05.116

Reaction

Chain : A
UniProt : Q50LF0 (TSOXA_CORS9)
Reaction: EC: Evidence:
Physiological Direction:
(6S)-5,6,7,8-tetrahydrofolate + O2 + sarcosine = (6R)-5,10- methylene-5,6,7,8-tetrahydrofolate + glycine + H2O2 1.5.3.24 PubMed:20675294, PubMed:15914915
-
H2O + O2 + sarcosine = formaldehyde + glycine + H2O2 - PubMed:17395614, PubMed:20675294, PubMed:7240129
-
Chain : B
UniProt : Q50LF2 (TSOXB_CORS9)
Reaction: EC: Evidence:
Physiological Direction:
(6S)-5,6,7,8-tetrahydrofolate + O2 + sarcosine = (6R)-5,10- methylene-5,6,7,8-tetrahydrofolate + glycine + H2O2 1.5.3.24 PubMed:20675294, PubMed:15914915
-
H2O + O2 + sarcosine = formaldehyde + glycine + H2O2 - PubMed:17395614, PubMed:20675294, PubMed:7240129
-
Chain : C
UniProt : Q50LE9 (TSOXG_CORS9)
Reaction: EC: Evidence:
Physiological Direction:
(6S)-5,6,7,8-tetrahydrofolate + O2 + sarcosine = (6R)-5,10- methylene-5,6,7,8-tetrahydrofolate + glycine + H2O2 1.5.3.24 PubMed:20675294, PubMed:15914915
-
H2O + O2 + sarcosine = formaldehyde + glycine + H2O2 - PubMed:17395614, PubMed:20675294, PubMed:7240129
-
Chain : D
UniProt : Q50LF1 (TSOXD_CORS9)
Reaction: EC: Evidence:
Physiological Direction:
(6S)-5,6,7,8-tetrahydrofolate + O2 + sarcosine = (6R)-5,10- methylene-5,6,7,8-tetrahydrofolate + glycine + H2O2 1.5.3.24 PubMed:20675294, PubMed:15914915
-
H2O + O2 + sarcosine = formaldehyde + glycine + H2O2 - PubMed:17395614, PubMed:20675294, PubMed:7240129
-