Brand  (β version)

PDB ID: 1UMD

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
COI 2-oxo-4-methylpentanoic acid PoSSuM
MG Magnesium ion PoSSuM
TPP Thiamine diphosphate PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 1UMD   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : branched-chain 2-oxo acid dehydrogenase (E1) from Thermus thermophilus HB8 with 4-methyl-2-oxopentanoate as an intermediate
Release Data : 2004-03-30
Compound :
mol_id molecule chains synonym
1 2-oxo acid dehydrogenase alpha subunit A,C E1-alpha
ec: 1.2.4.4
mol_id molecule chains synonym
2 2-oxo acid dehydrogenase beta subunit B,D E1-beta
ec: 1.2.4.4
Source :
mol_id organism_scientific expression_system
1 Thermus thermophilus  (taxid:274) Escherichia coli  (taxid:562)
expression_system_strain: BLR(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pACYC184
mol_id organism_scientific expression_system
2 Thermus thermophilus  (taxid:274) Escherichia coli  (taxid:562)
expression_system_strain: BLR(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET11A
Authors : Nakai, T., Nakagawa, N., Maoka, N., Masui, R., Kuramitsu, S., Kamiya, N., RIKEN Structural Genomics/Proteomics Initiative (RSGI)
Keywords : alpha(2)beta(2) tetramer, structural genomics, RIKEN Structural Genomics/Proteomics Initiative, RSGI, Oxidoreductase
Exp. method : X-RAY DIFFRACTION ( 1.90 Å )
Citation :

Ligand-induced Conformational Changes and a Reaction Intermediate in Branched-chain 2-Oxo Acid Dehydrogenase (E1) from Thermus thermophilus HB8, as Revealed by X-ray Crystallography

Nakai, T.,Nakagawa, N.,Maoka, N.  et al.
(2004)  J.Mol.Biol.  337 : 1011 - 1033

PubMed: 15033367
DOI: 10.1016/j.jmb.2004.02.011

Reaction

Chain : A, C
UniProt : Q5SLR4 (ODBA_THET8)
Reaction: EC: Evidence:
Physiological Direction:
3-methyl-2-oxobutanoate + H(+) + N(6)-[(R)-lipoyl]-L-lysyl- [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] = CO2 + N(6)-[(R)-S(8)-2-methylpropanoyldihydrolipoyl]-L-lysyl- [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] 1.2.4.4 PubMed:15033367
-
Chain : B, D
UniProt : Q5SLR3 (ODBB_THET8)
Reaction: EC: Evidence:
Physiological Direction:
3-methyl-2-oxobutanoate + H(+) + N(6)-[(R)-lipoyl]-L-lysyl- [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] = CO2 + N(6)-[(R)-S(8)-2-methylpropanoyldihydrolipoyl]-L-lysyl- [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] 1.2.4.4 PubMed:15033367, ECO:0000305
-