Brand  (β version)

PDB ID: 1UA0

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Ligands
Code Name Style Show Link
AF 2-aminofluorene PoSSuM
SO4 Sulfate ion PoSSuM
FRU Beta-D-fructofuranose PoSSuM
GLC Alpha-D-glucopyranose PoSSuM
: Polysaccharide
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Structure summary

Code : 1UA0   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/DNA
Title : Aminofluorene DNA adduct at the pre-insertion site of a DNA polymerase
Release Data : 2004-09-28
Compound :
mol_id molecule chains
1 DNA primer strand B
mol_id molecule chains
2 DNA template strand with aminofluorene adduct C
other_details: see remark 400
mol_id molecule chains synonym
3 DNA polymerase I A POL I
ec: 2.7.7.7
fragment: analogous to the E. coli klenow fragment
other_details: see remark 400
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific expression_system
3 Geobacillus stearothermophilus  (taxid:1422) Escherichia coli  (taxid:562)
expression_system_strain: BL21(DE3)PlysS
expression_system_vector_type: plasmid
expression_system_plasmid: pet-30A(+)
Authors : Hsu, G.W., Kiefer, J.R., Becherel, O.J., Fuchs, R.P.P., Beese, L.S.
Keywords : DNA polymerase I; DNA replication; klenow fragment; protein-DNA complex; aminofluorene; aromatic amine; DNA lesion; translation replication, TRANSFERASE-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.10 Å )
Citation :

Observing translesion synthesis of an aromatic amine DNA adduct by a high-fidelity DNA polymerase

Hsu, G.W.,Kiefer, J.R.,Burnouf, D.  et al.
(2004)  J.Biol.Chem.  279 : 50280 - 50285

PubMed: 15385534
DOI: 10.1074/jbc.M409224200

Structures of Mismatch Replication Errors Observed in a DNA Polymerase

Johnson, S.J.,Beese, L.S.
(2004)  Cell(Cambridge,Mass.)  116 : 803 - 816

Processive DNA synthesis observed in a polymerase crystal suggests a mechanism for the prevention of frameshift mutations

Johnson, S.J.,Taylor, J.S.,Beese, L.S.
(2003)  Proc.Natl.Acad.Sci.USA  100 : 3895 - 3900

Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal

Kiefer, J.R.,Mao, C.,Braman, J.C.  et al.
(1998)  Nature  391 : 304 - 307