Brand  (β version)

PDB ID: 1U0H

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Ligands
Code Name Style Show Link
CL Chloride ion PoSSuM
FOK Forskolin PoSSuM
GSP 5'-guanosine-diphosphate-monothiophosphate PoSSuM
MG Magnesium ion PoSSuM
ONM 3'-O-(N-methylanthraniloyl)-guanosine-5'-triphosphate PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1U0H   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : STRUCTURAL BASIS FOR THE INHIBITION OF MAMMALIAN ADENYLYL CYCLASE BY MANT-GTP
Release Data : 2004-12-14
Compound :
mol_id molecule chains synonym
1 Adenylate cyclase, type V A ATP pyrophosphate-lyase, Ca2+, -inhibitable adenylyl cyclase
ec: 4.6.1.1
fragment: C1A domain of Adenylyl Cyclase
mol_id molecule chains synonym
2 Adenylate cyclase, type II B ATP pyrophosphate-lyase, Adenylyl cyclase
ec: 4.6.1.1
fragment: C2A domain of Adenylyl Cyclase
mol_id molecule chains synonym
3 Guanine nucleotide-binding protein G(s), alpha subunit C Adenylate cyclase-stimulating G alpha protein
Source :
mol_id organism_scientific organism_common expression_system
1 Canis lupus familiaris  (taxid:9615) Dog Escherichia coli BL21  (taxid:511693)
strain: familiaris
gene: ADCY5;
expression_system_strain: BL21
expression_system_vector_type: Multi-copy plasmid
expression_system_plasmid: PQE60-H6-VC1(580)
mol_id organism_scientific organism_common expression_system
2 Rattus norvegicus  (taxid:10116) Norway rat Escherichia coli BL21  (taxid:511693)
gene: Adcy2;
expression_system_strain: BL21
expression_system_vector_type: Multi-copy plasmid
expression_system_plasmid: PQE60-ARGC-IIC2
mol_id organism_scientific organism_common expression_system
3 Bos taurus  (taxid:9913) Cattle Escherichia coli BL21  (taxid:511693)
gene: GNAS;
expression_system_strain: BL21
expression_system_vector_type: Multi-copy plasmid
expression_system_plasmid: PQE60-GSALPHA-H
Authors : Mou, T.C., Gille, A., Seifert, R.J., Sprang, S.R.
Keywords : adenylyl cyclase, Gsa, MANT-GTP, LYASE
Exp. method : X-RAY DIFFRACTION ( 2.90 Å )
Citation :

Structural basis for the inhibition of mammalian membrane adenylyl cyclase by 2 '(3')-O-(N-Methylanthraniloyl)-guanosine 5 '-triphosphate.

Mou, T.C.,Gille, A.,Fancy, D.A.  et al.
(2005)  J.Biol.Chem.  280 : 7253 - 7261

PubMed: 15591060
DOI: 10.1074/jbc.M409076200

Reaction

Chain : A
UniProt : P30803 (ADCY5_CANLF)
Reaction: EC: Evidence:
Physiological Direction:
ATP = 3',5'-cyclic AMP + diphosphate 4.6.1.1 PubMed:10427002, PubMed:11087399, PubMed:15591060, PubMed:1618857, PubMed:16766715, PubMed:19243146, PubMed:8428899
-
Chain : B
UniProt : P26769 (ADCY2_RAT)
Reaction: EC: Evidence:
Physiological Direction:
ATP = 3',5'-cyclic AMP + diphosphate 4.6.1.1 PubMed:10427002, PubMed:11087399, PubMed:15591060, PubMed:16766715, PubMed:1719547, PubMed:19243146, PubMed:21596131, PubMed:22906005, PubMed:24363043, PubMed:7761832
-
Chain : C
UniProt : P04896 (GNAS2_BOVIN)
Reaction : -