Brand  (β version)

PDB ID: 1TW6

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Ligands
Code Name Style Show Link
ZN Zinc ion PoSSuM
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Glycosylation
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Structure summary

Code : 1TW6   PDBj   RCSB PDB   PDBe
Header : INHIBITOR/APOPTOSIS
Title : Structure of an ML-IAP/XIAP chimera bound to a 9mer peptide derived from Smac
Release Data : 2004-11-02
Compound :
mol_id molecule chains synonym
1 Baculoviral IAP repeat-containing protein 7 A,B Kidney inhibitor of apoptosis protein, KIAP, Melanoma inhibitor of apoptosis protein, ML-IAP, Livin, UNQ5800/PRO19607/PRO21344, XIAP-BIR3 chimera
fragment: ML-IAP residues 63-172
mutation: S150G, R160G, D161E, F162Y, V163I, H164N, S165N, V166I, Q167H, E168L, Q172L
other_details: residues 150, 160-168, and 172 replaced with XIAP-BIR3 homologues
mol_id molecule chains synonym
2 Diablo homolog, mitochondrial C,D Second mitochondria-derived activator of caspase, Smac protein, Direct IAP binding protein with low pI
fragment: Smac residues 1-9
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli BL21(DE3)  (taxid:469008)
gene: BIRC7
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET15b
mol_id organism_scientific organism_common expression_system
2 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: DIABLO, SMAC
Authors : Franklin, M.C., Vucic, D., Wallweber, H.J.A., Das, K., Shin, H., Elliott, L.O., Kadkhodayan, S., Deshayes, K., Salvesen, G.S., Fairbrother, W.J.
Keywords : ZINC BINDING, PEPTIDE COMPLEX, APOPTOSIS INHIBITION, INHIBITOR-APOPTOSIS COMPLEX
Exp. method : X-RAY DIFFRACTION ( 1.713 Å )
Citation :

Engineering ML-IAP to produce an extraordinarily potent caspase 9 inhibitor: implications for Smac-dependent anti-apoptotic activity of ML-IAP

Vucic, D.,Franklin, M.C.,Wallweber, H.J.A.  et al.
(2005)  Biochem.J.  385 : 11 - 20

PubMed: 15485396
DOI: 10.1042/BJ20041108

Structure and Function Analysis of Peptide Antagonists of Melanoma Inhibitor of Apoptosis (ML-IAP)

Franklin, M.C.,Kadkhodayan, S.,Ackerly, H.  et al.
(2003)  Biochemistry  42 : 8223 - 8231

PubMed: 12846571
DOI: 10.1021/bi034227t

Mechanism of XIAP-mediated inhibition of caspase-9

Shizoaki, E.N.,Chai, J.,Rigotti, D.J.  et al.
(2003)  Mol.Cell  11 : 519 - 527

PubMed: 12620238
DOI: 10.1016/S1097-2765(03)00054-6

Reaction

Chain : A, B
UniProt : Q96CA5 (BIRC7_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. 2.3.2.27 PubMed:16729033
-
Chain : C, D
UniProt : Q9NR28 (DBLOH_HUMAN)
Reaction : -