Brand  (β version)

PDB ID: 1TW5

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Ligands
Code Name Style Show Link
MN Manganese (II) ion PoSSuM
UDH 6-aminohexyl-uridine-c1,5'-diphosphate PoSSuM
SO4 Sulfate ion PoSSuM
DIO 1,4-diethylene dioxide PoSSuM
MES 2-(N-morpholino)-ethanesulfonic acid PoSSuM
NAG 2-acetamido-2-deoxy-beta-D-glucopyranose PoSSuM
: Polysaccharide
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Structure summary

Code : 1TW5   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : beta1,4-galactosyltransferase mutant M344H-Gal-T1 in complex with Chitobiose
Release Data : 2004-12-14
Compound :
mol_id molecule chains synonym
1 Beta-1,4-galactosyltransferase 1 A,B Beta-1,4-GalTase 1; Beta4Gal-T1; b4Gal-T1
ec: 2.4.1.22, 2.4.1.90, 2.4.1.38
fragment: catalytic domain
mutation: d129, Cys342Thr and Met344His
Source :
mol_id organism_scientific organism_common expression_system
1 Bos taurus  (taxid:9913) Cattle Escherichia coli BL21  (taxid:511693)
gene: B4GALT1
expression_system_strain: BL21
expression_system_vector_type: Plasmid
expression_system_plasmid: pET23a
other_details: N-terminal 13 amino acids belong to T7-tag from the vector
Authors : Ramakrishnan, B., Boeggeman, E., Qasba, P.K.
Keywords : Met344His mutation; closed conformation; chitobiose binding, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.30 Å )
Citation :

Effect of the Met344His mutation on the conformational dynamics of bovine beta-1,4-galactosyltransferase: crystal structure of the Met344His mutant in complex with chitobiose

Ramakrishnan, B.,Boeggeman, E.,Qasba, P.K.
(2004)  Biochemistry  43 : 12513 - 12522

PubMed: 15449940
DOI: 10.1021/bi049007+

Reaction

Chain : A, B
UniProt : P08037 (B4GT1_BOVIN)
Reaction: EC: Evidence:
Physiological Direction:
D-glucose + UDP-alpha-D-galactose = lactose + UDP + H(+) 2.4.1.22 PubMed:12927542, PubMed:9405390
left-to-right PubMed:9405390, PubMed:12927542
an N-acetyl-beta-D-glucosaminyl derivative + UDP-alpha-D- galactose = a beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative + UDP + H(+) 2.4.1.38 PubMed:9405390
left-to-right
N-acetyl-D-glucosamine + UDP-alpha-D-galactose = beta-D- galactosyl-(1->4)-N-acetyl-D-glucosamine + UDP + H(+) 2.4.1.90 PubMed:9405390
left-to-right
a beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)- Cer(d18:1(4E)) + UDP-alpha-D-galactose = a neolactoside nLc4Cer(d18:1(4E)) + UDP + H(+) 2.4.1.275 PubMed:9405390
left-to-right
a beta-D-glucosylceramide + UDP-alpha-D-galactose = a beta-D- galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide + UDP + H(+) - PubMed:9405390
left-to-right
a neolactoside IV(3)-beta-GlcNAc-nLc4Cer + UDP-alpha-D- galactose = a neolactoside nLc6Cer + UDP + H(+) - PubMed:9405390
left-to-right
Cofactor: Evidence: Note:
Mn(2+) ECO:0000250 | UniProtKB:P15291
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