Brand  (β version)

PDB ID: 1TK5

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Ligands
Code Name Style Show Link
1PE Pentaethylene glycol PoSSuM
MES 2-(N-morpholino)-ethanesulfonic acid PoSSuM
MG Magnesium ion PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
Code Name Show
8OG 8-oxo-2'-deoxy-guanosine-5'-monophosphate
DDG 2',3'-dideoxy-guanosine-5'-monophosphate
Glycosylation
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Modification
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Structure summary

Code : 1TK5   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/ELECTRON TRANSPORT/DNA
Title : T7 DNA polymerase binary complex with 8 oxo guanosine in the templating strand
Release Data : 2004-08-31
Compound :
mol_id molecule chains
1 5'-D(*CP*GP*AP*AP*A*GP*CP*CP*AP*GP*TP*GP*CP*CP*AP*(DDG)P*TP*GP*CP*AP*A)-3' P
mol_id molecule chains
2 5'-D(*CP*CP*CP*(8OG)P*CP*TP*GP*GP*CP*AP*CP*TP*GP*GP*CP*CP*GP*TP*CP*GP*TP*TP*TP*TP*CP*G)-3' T
mol_id molecule chains synonym
3 DNA polymerase A T7 DNA polymerase
ec: 2.7.7.7
mol_id molecule chains synonym
4 Thioredoxin 1 B TRX1, TRX
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific expression_system
3 Enterobacteria phage T7  (taxid:10760) Escherichia coli BL21  (taxid:511693)
gene: 5
expression_system_strain: BL21
expression_system_plasmid: pGP5
mol_id organism_scientific expression_system
4 Escherichia coli  (taxid:562) Escherichia coli BL21  (taxid:511693)
gene: TRXA, TSNC, FIPA, B3781, C4701, Z5291, ECS4714, STM3915, STMD1.75, STY3639, T3381, SF3854, S3905
expression_system_strain: BL21
expression_system_plasmid: pTrx-3
Authors : Brieba, L.G., Eichman, B.F., Kokoska, R.J., Doublie, S., Kunkel, T.A., Ellenberger, T.
Keywords : 8-oxoguanosine DNA polymerase, TRANSFERASE-ELECTRON TRANSPORT-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.20 Å )
Citation :

Structural basis for the dual coding potential of 8-oxoguanosine by a high-fidelity DNA polymerase.

Brieba, L.G.,Eichman, B.F.,Kokoska, R.J.  et al.
(2004)  Embo J.  23 : 3452 - 3461

PubMed: 15297882
DOI: 10.1038/sj.emboj.7600354

Reaction

Chain : A
UniProt : P00581 (DPOL_BPT7)
Reaction: EC: Evidence:
Physiological Direction:
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 2.7.7.7 HAMAP-Rule:MF_04101, PubMed:24591606
-
Cofactor: Evidence: Note:
Mg(2+) ECO:0000255 | HAMAP-Rule:MF_04101
ECO:0000305 | PubMed:9440688
-
Chain : B
UniProt : P0AA25 (THIO_ECOLI)
Reaction : -