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Ligands
Code Name Style Show Link
1PE Pentaethylene glycol
DCT 2',3'-dideoxycytidine 5'-triphosphate
MES 2-(N-morpholino)-ethanesulfonic acid
MG Magnesium ion
SO4 Sulfate ion
Non-standard Residues
Code Name Show
8OG 8-oxo-2'-deoxy-guanosine-5'-monophosphate
DDG 2',3'-dideoxy-guanosine-5'-monophosphate
Glycosylation
Code Name Emphasize
Modification
Code Name Show
Code : 1TK0   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/ELECTRON TRANSPORT/DNA
Title : T7 DNA polymerase ternary complex with 8 oxo guanosine and ddCTP at the insertion site
Release Data : 2004-08-31
Compound :
mol_id molecule chains
1 5'-D(*CP*GP*AP*AP*AP*AP*CP*GP*A*CP*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*AP*(DDG))-3' P
other_details: primer strand
mol_id molecule chains
2 5'-D(*CP*CP*CP*(8OG)P*CP*TP*GP*GP*CP*AP*CP*TP*GP*GP*CP*CP*GP*TP*CP*GP*TP*TP*TP*TP*CP*G)-3' T
other_details: template strand
mol_id molecule chains synonym
3 DNA polymerase A T7 DNA polymerase
ec: 2.7.7.7
mutation: deletion 118-123
mol_id molecule chains synonym
4 Thioredoxin 1 B TRX1, TRX
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific expression_system
3 Enterobacteria phage T7  (taxid:10760) Escherichia coli BL21  (taxid:511693)
gene: 5
expression_system_strain: BL-21
expression_system_plasmid: pGP5
mol_id organism_scientific expression_system
4 Escherichia coli  (taxid:562) Escherichia coli BL21  (taxid:511693)
gene: TRXA, TSNC, FIPA, B3781, C4701, Z5291, ECS4714, STM3915, STMD1.75, STY3639, T3381, SF3854, S3905
expression_system_strain: BL21
expression_system_vector_type: pTrx-3
Authors : Brieba, L.G., Eichman, B.F., Kokoska, R.J., Doublie, S., Kunkel, T.A., Ellenberger, T.
Keywords : 8 oxo guanosine, DNA polymerase, TRANSFERASE-ELECTRON TRANSPORT-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.30 Å )
Citation :

Structural basis for the dual coding potential of 8-oxoguanosine by a high-fidelity DNA polymerase.

Brieba, L.G.,Eichman, B.F.,Kokoska, R.J.  et al.
(2004)  Embo J.  23 : 3452 - 3461

PubMed: 15297882
DOI: 10.1038/sj.emboj.7600354

Chain : A
UniProt : P00581 (DPOL_BPT7)
Reaction: EC: Evidence:
Physiological Direction:
a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 2.7.7.7 HAMAP- Rule:MF_04101, PubMed:24591606
-
Chain : B
UniProt : P0AA25 (THIO_ECOLI)
Reaction : -