Brand  (β version)

PDB ID: 1T9D

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Ligands
Code Name Style Show Link
K Potassium ion PoSSuM
MG Magnesium ion PoSSuM
1MM Methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate PoSSuM
FAD Flavin-adenine dinucleotide PoSSuM
PYD 2,5-dimethyl-pyrimidin-4-ylamine PoSSuM
P25 Pentyl trihydrogen diphosphate PoSSuM
Non-standard Residues
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Structure summary

Code : 1T9D   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal Structure Of Yeast Acetohydroxyacid Synthase In Complex With A Sulfonylurea Herbicide, Metsulfuron methyl
Release Data : 2004-12-21
Compound :
mol_id molecule chains synonym
1 Acetolactate synthase, mitochondrial A,B,C,D Acetohydroxy-acid synthase, ALS, AHAS
ec: 2.2.1.6
fragment: Catalytic Subunit
Source :
mol_id organism_scientific organism_common expression_system
1 Saccharomyces cerevisiae  (taxid:4932) Baker's yeast Escherichia coli BL21(DE3)  (taxid:469008)
gene: ILV2, SMR1, YMR108W, YM9718.07
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET30(c)
Authors : McCourt, J.A., Pang, S.S., Guddat, L.W., Duggleby, R.G.
Keywords : acetohydroxyacid synthase, acetolactate synthase, herbicide, sulfonylurea, thiamin diphosphate, FAD, inhibitor, metsulfuron methyl, transferase
Exp. method : X-RAY DIFFRACTION ( 2.30 Å )
Citation :

Elucidating the specificity of binding of sulfonylurea herbicides to acetohydroxyacid synthase.

McCourt, J.A.,Pang, S.S.,Guddat, L.W.  et al.
(2005)  Biochemistry  44 : 2330 - 2338

PubMed: 15709745
DOI: 10.1021/bi047980a

Reaction

Chain : A, B, C, D
UniProt : P07342 (ILVB_YEAST)
Reaction: EC: Evidence:
Physiological Direction:
H(+) + 2 pyruvate = (2S)-2-acetolactate + CO2 2.2.1.6 PubMed:10213630
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2-oxobutanoate + H(+) + pyruvate = (S)-2-ethyl-2-hydroxy-3- oxobutanoate + CO2 2.2.1.6 PubMed:10213630
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