Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
FLV Flaviolin
HEM Protoporphyrin ix containing Fe
Code : 1T93
Header : OXIDOREDUCTASE
Title : Evidence for Multiple Substrate Recognition and Molecular Mechanism of C-C reaction by Cytochrome P450 CYP158A2 from Streptomyces Coelicolor A3(2)
Release Data : 2005-01-25
Compound :
mol_id molecule chains
1 putative cytochrome P450 A
Source :
mol_id organism_scientific expression_system
1 Streptomyces coelicolor  (taxid:100226) Escherichia coli  (taxid:562)
strain: A3(2)
expression_system_strain: HMS174(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET17b
Authors : Zhao, B., Sundaramoorthy, M., Waterman, M.R.
Keywords : streptomyces, Cytochrome P450 oxidoreductase, CYP158A2, substrate recognition, molecular mechanism, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 1.62 Å )
Citation :

Binding of Two Flaviolin Substrate Molecules, Oxidative Coupling, and Crystal Structure of Streptomyces coelicolor A3(2) Cytochrome P450 158A2.

Zhao, B.,Guengerich, F.P.,Bellamine, A.  et al.
(2005)  J.Biol.Chem.  280 : 11599 - 11607

PubMed: 15659395
DOI: 10.1074/jbc.M410933200

Chain : A
UniProt : Q9FCA6 (C1582_STRCO)
Reaction : 2 flaviolin + NADPH + O(2) = 3,3'-biflaviolin + NADP(+) + 2 H(2)O.
2 flaviolin + NADPH + O(2) = 3,8'-biflaviolin + NADP(+) + 2 H(2)O.