Brand  (β version)

PDB ID: 1T8A

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
CL Chloride ion PoSSuM
GAI Guanidine PoSSuM
HED 2-hydroxyethyl disulfide PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 1T8A   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : USE OF SEQUENCE DUPLICATION TO ENGINEER A LIGAND-TRIGGERED LONG-DISTANCE MOLECULAR SWITCH IN T4 Lysozyme
Release Data : 2004-08-17
Compound :
mol_id molecule chains synonym
1 Lysozyme A Lysis protein, Muramidase, Endolysin
ec: 3.2.1.17
mutation: L39I, R63A, C54T,C97A
Source :
mol_id organism_scientific expression_system
1 Enterobacteria phage T4  (taxid:10665) Escherichia coli  (taxid:562)
gene: E
Authors : Yousef, M.S., Baase, W.A., Matthews, B.W.
Keywords : MOLECULAR SWITCH, T4 LYSOZYME, NANO-BITECHNOLOGY, PROTEIN ENGINEERING, PROTEIN DESIGN, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 2.00 Å )
Citation :

Use of sequence duplication to engineer a ligand-triggered, long-distance molecular switch in T4 lysozyme.

Yousef, M.S.,Baase, W.A.,Matthews, B.W.
(2004)  Proc.Natl.Acad.Sci.USA  101 : 11583 - 11586

PubMed: 15286283
DOI: 10.1073/pnas.0404482101

Structural characterization of an engineered tandem repeat contrasts the importance of context and sequence in protein folding.

Sagermann, M.,Baase, W.A.,Matthews, B.W.
(1999)  Proc.Natl.Acad.Sci.USA  96 : 6078 - 6083

PubMed: 10339544
DOI: 10.1073/pnas.96.11.6078

Long-Distance Conformational Changes in a Protein Engineered by Modulated Sequence Duplication

Sagermann, M.,Gay, L.,Matthews, B.W.
(2003)  Proc.Natl.Acad.Sci.USA  100 : 9191 - 9195

PubMed: 12869697
DOI: 10.1073/pnas.1633549100

Reaction

Chain : A
UniProt : P00720 (ENLYS_BPT4)
Reaction: EC: Evidence:
Physiological Direction:
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. 3.2.1.17 HAMAP-Rule:MF_04110, PubMed:4865643
-