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Ligands
Code Name Style Show Link
CAM Camphor
CMO Carbon monoxide
HEM Protoporphyrin ix containing Fe
K Potassium ion
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Code : 1T85   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Crystal Structure of the Ferrous CO-bound Cytochrome P450cam Mutant (L358P/C334A)
Release Data : 2004-06-01
Compound :
mol_id molecule chains synonym
1 Cytochrome P450-cam A Camphor 5-monooxygenase, P450cam
ec: 1.14.15.1
mutation: L358P,C334A
Source :
mol_id organism_scientific expression_system
1 Pseudomonas putida  (taxid:303) Escherichia coli BL21  (taxid:511693)
gene: CAMC, CYP101
expression_system_strain: BL21
expression_system_vector_type: plasmid
expression_system_plasmid: pT7
Authors : Nagano, S., Tosha, T., Ishimori, K., Morishima, I., Poulos, T.L.
Keywords : cytochrome P450, oxidoreductase, heme enzyme
Exp. method : X-RAY DIFFRACTION ( 1.80 Å )
Citation :

Crystal structure of the cytochrome p450cam mutant that exhibits the same spectral perturbations induced by putidaredoxin binding.

Nagano, S.,Tosha, T.,Ishimori, K.  et al.
(2004)  J.Biol.Chem.  279 : 42844 - 42849

PubMed: 15269210
DOI: 10.1074/jbc.M404217200

Chain : A
UniProt : P00183 (CPXA_PSEPU)
Reaction: EC: Evidence:
Physiological Direction:
(1R,4R)-camphor + 2 H(+) + O2 + 2 reduced [2Fe-2S]- [putidaredoxin] = (1R,4R,5R)-5-hydroxycamphor + H2O + 2 oxidized [2Fe-2S]-[putidaredoxin] 1.14.15.1 -
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