Brand  (β version)

PDB ID: 1T36

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Ligands
Code Name Style Show Link
ADP Adenosine-5'-diphosphate PoSSuM
CL Chloride ion PoSSuM
K Potassium ion PoSSuM
MN Manganese (II) ion PoSSuM
NET Tetraethylammonium ion PoSSuM
ORN L-ornithine PoSSuM
PO4 Phosphate ion PoSSuM
U Uridine-5'-monophosphate PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1T36   PDBj   RCSB PDB   PDBe
Header : LIGASE
Title : Crystal structure of E. coli carbamoyl phosphate synthetase small subunit mutant C248D complexed with uridine 5'-monophosphate
Release Data : 2004-09-21
Compound :
mol_id molecule chains synonym
1 Carbamoyl-phosphate synthase large chain A,C,E,G Carbamoyl-phosphate synthetase ammonia chain
ec: 6.3.5.5
mol_id molecule chains synonym
2 Carbamoyl-phosphate synthase small chain B,D,F,H Carbamoyl-phosphate synthetase glutamine chain
ec: 6.3.5.5
mutation: C248D
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli BL21  (taxid:511693)
gene: CARB, PYRA, B0033
expression_system_strain: BL21
expression_system_vector_type: plasmid
expression_system_plasmid: pet15
mol_id organism_scientific expression_system
2 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
gene: CARA, PYRA, B0032, Z0037, ECS0035
expression_system_vector_type: plasmid
expression_system_plasmid: pet15
Authors : Thoden, J.B., Huang, X., Raushel, F.M., Holden, H.M.
Keywords : channeling, pyrimidine biosynthesis, arginine biosynthesis, LIGASE
Exp. method : X-RAY DIFFRACTION ( 2.10 Å )
Citation :

Long-range allosteric transitions in carbamoyl phosphate synthetase.

Thoden, J.B.,Huang, X.,Kim, J.  et al.
(2004)  Protein Sci.  13 : 2398 - 2405

PubMed: 15322282
DOI: 10.1110/ps.04822704

Reaction

Chain : A, C, E, G
UniProt : P00968 (CARB_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate 6.3.5.5 HAMAP- Rule:MF_01210, PubMed:4944634
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[Carbamoyl phosphate synthase large chain]
2 ATP + hydrogencarbonate + NH4(+) = 2 ADP + carbamoyl phosphate + 2 H(+) + phosphate 		6.3.4.16 HAMAP- Rule:MF_01210, PubMed:4944634
-
Chain : B, D, F, H
UniProt : P00907 (CARA_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate 6.3.5.5 HAMAP- Rule:MF_01209, PubMed:4944634
-
[Carbamoyl phosphate synthase small chain]
H2O + L-glutamine = L-glutamate + NH4(+) 		- HAMAP- Rule:MF_01209, PubMed:4944634
-