Brand  (β version)

PDB ID: 1SZO

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Ligands
Code Name Style Show Link
CAX (2s,4s)-4-(2,2-dihydroxyethyl)-2,3,3-trimethylcyclopentanone PoSSuM
CA Calcium ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1SZO   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Crystal Structure Analysis of the 6-Oxo Camphor Hydrolase His122Ala Mutant Bound to Its Natural Product (2S,4S)-alpha-Campholinic Acid
Release Data : 2004-06-29
Compound :
mol_id molecule chains
1 6-oxocamphor hydrolase A,B,C,D,E,F,G,H,I,J,K,L
mutation: H122A
Source :
mol_id organism_scientific expression_system
1 Rhodococcus sp. NCIMB 9784  (taxid:157732) Escherichia coli BL21(DE3)  (taxid:469008)
gene: camK
expression_system_strain: BL21 (DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET-26b (Novagen)
Authors : Leonard, P.M., Grogan, G.
Keywords : Enzyme-Product Complex, hydrolase
Exp. method : X-RAY DIFFRACTION ( 1.9 Å )
Citation :

Structure of 6-oxo camphor hydrolase H122A mutant bound to its natural product, (2S,4S)-alpha-campholinic acid: mutant structure suggests an atypical mode of transition state binding for a crotonase homolog.

Leonard, P.M.,Grogan, G.
(2004)  J.Biol.Chem.  279 : 31312 - 31317

PubMed: 15138275
DOI: 10.1074/jbc.M403514200

The 2- Crystal Structure of 6-Oxo Camphor Hydrolase. NEW STRUCTURAL DIVERSITY IN THE CROTONASE SUPERFAMILY.

Whittingham, J.L.,Turkenburg, J.P.,Verma, C.S.  et al.
(2003)  J.Biol.Chem.  278 : 1744 - 1750

DOI: 10.1074/jbc.M211188200

The Desymmetrization of Bicyclic beta-Diketones by an Enzymatic Retro-Claisen Reaction. A NEW REACTION OF THE CROTONASE SUPERFAMILY.

Grogan, G.,Roberts, G.A.,Bougioukou, D.  et al.
(2001)  J.Biol.Chem.  276 : 12565 - 12572

DOI: 10.1074/jbc.M011538200

An Asymmetric Enzyme-Catalysed Retro-Claisen Reaction for the Desymmetrisation of Cyclic beta-Diketones.

Grogan, G.,Graf, J.,Jones, A.  et al.
(2001)  Angew.Chem.Int.Ed.Engl.  40 : 1111 - 1114

DOI: 10.1002/1521-3773(20010316)40:6<1111::AID-ANIE11110>3.3.CO;2-U

Reaction

Chain : A, B, C, D, E, F, G, H, I, J, K, L
UniProt : Q93TU6 (CAMK_RHOSO)
Reaction: EC: Evidence:
Physiological Direction:
bornane-2,6-dione + H2O = [(1S)-4-hydroxy-2,2,3- trimethylcyclopent-3-enyl]acetate + H(+) 3.7.1.18 PubMed:11278926
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