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Ligands
Code Name Style Show Link
FNS N-sulfo-flavin mononucleotide
Non-standard Residues
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Glycosylation
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Code : 1SZG   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : A198G:L230A flavocytochrome b2 with sulfite bound
Release Data : 2004-07-27
Compound :
mol_id molecule chains synonym
1 Cytochrome b2, mitochondrial A,B L-lactate dehydrogenase [Cytochrome]; L-lactate ferricytochrome C oxidoreductase; L-LCR
ec: 1.1.2.3
fragment: residues 81-591
mutation: A198G:L230A
Source :
mol_id organism_scientific organism_common expression_system
1 Saccharomyces cerevisiae  (taxid:4932) Baker's yeast Escherichia coli  (taxid:562)
gene: CYB2
expression_system_strain: TG1
expression_system_vector_type: plasmid
expression_system_plasmid: pDSb2
Authors : Mowat, C.G., Wehenkel, A., Green, A.J., Walkinshaw, M.D., Reid, G.A., Chapman, S.K.
Keywords : flavocytochrome; L-lactate dehydrogenase; sulfite, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 2.70 Å )
Citation :

Altered Substrate Specificity in Flavocytochrome b(2): Structural Insights into the Mechanism of l-Lactate Dehydrogenation

Mowat, C.G.,Wehenkel, A.,Green, A.J.  et al.
(2004)  Biochemistry  43 : 9519 - 9526

PubMed: 15260495
DOI: 10.1021/bi049263m

Chain : A, B
UniProt : P00175 (CYB2_YEAST)
Reaction: EC: Evidence:
Physiological Direction:
(S)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + 2 H(+) + pyruvate 1.1.2.3 PubMed:11914072, PubMed:3004948, PubMed:4593578
left-to-right PubMed:3004948