Brand  (β version)

PDB ID: 1S6Z

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Ligands
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CL Chloride ion PoSSuM
Non-standard Residues
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CR0 [2-(1-amino-2-hydroxypropyl)-2-hydroxy-4-isobutyl-5-oxo-2,5-dihydro-1h-imidazol-1-yl]acetaldehyde
Glycosylation
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Structure summary

Code : 1S6Z   PDBj   RCSB PDB   PDBe
Header : LUMINESCENT PROTEIN
Title : Enhanced Green Fluorescent Protein Containing the Y66L Substitution
Release Data : 2004-05-04
Compound :
mol_id molecule chains
1 green fluorescent protein A
mutation: F64L/S65T/Y66L
Source :
mol_id organism_scientific expression_system
1 Synthetic construct  (taxid:32630) Escherichia coli  (taxid:562)
expression_system_strain: JM109-DE3
expression_system_vector_type: plasmid
expression_system_plasmid: pRSETB
Authors : Rosenow, M.A., Huffman, H.A., Phail, M.E., Wachter, R.M.
Keywords : Chromophore, Eleven-stranded Beta Barrel, Trapped Intermediate, Backbone cyclization, GFP-like Protein, LUMINESCENT PROTEIN
Exp. method : X-RAY DIFFRACTION ( 1.50 Å )
Citation :

The Crystal Structure of the Y66L Variant of Green Fluorescent Protein Supports a Cyclization-Oxidation-Dehydration Mechanism for Chromophore Maturation(,).

Rosenow, M.A.,Huffman, H.A.,Phail, M.E.  et al.
(2004)  Biochemistry  43 : 4464 - 4472

PubMed: 15078092
DOI: 10.1021/bi0361315