Brand  (β version)

PDB ID: 1S36

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
CEI N-[3-benzyl-5-(4-hydroxyphenyl)pyrazin-2-yl]-2-(4-hydroxyphenyl)acetamide PoSSuM
CL Chloride ion PoSSuM
GOL Glycerol PoSSuM
NA Sodium ion PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 1S36   PDBj   RCSB PDB   PDBe
Header : LUMINESCENT PROTEIN
Title : Crystal structure of a Ca2+-discharged photoprotein: Implications for the mechanisms of the calcium trigger and the bioluminescence
Release Data : 2004-10-05
Compound :
mol_id molecule chains synonym
1 Obelin A OBL
mutation: W92F
Source :
mol_id organism_scientific expression_system
1 Obelia longissima  (taxid:32570) Escherichia coli  (taxid:562)
expression_system_strain: BL21-Gold(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET19b+
Authors : Deng, L., Markova, S.V., Vysotski, E.S., Liu, Z.-J., Lee, J., Rose, J., Wang, B.-C., Southeast Collaboratory for Structural Genomics (SECSG)
Keywords : Photoprotein, obelin, bioluminescence, calcium binding, EF-hand, aequorin, Structural Genomics, PSI, Protein Structure Initiative, Southeast Collaboratory for Structural Genomics, SECSG, LUMINESCENT PROTEIN
Exp. method : X-RAY DIFFRACTION ( 1.96 Å )
Citation :

Crystal structure of a Ca2+-discharged photoprotein: implications for mechanisms of the calcium trigger and bioluminescence

Deng, L.,Markova, S.V.,Vysotski, E.S.  et al.
(2004)  J.Biol.Chem.  279 : 33647 - 33652

PubMed: 15155735
DOI: 10.1074/jbc.M402427200

Structure of the Ca2+-regulated photoprotein obelin at 1.7 resolution determined directly from its sulfur substructure

Liu, Z.-J.,Vysotski, E.S.,Chen, C.-J.  et al.
(2000)  Protein Sci.  9 : 2085 - 2093

Preparation and preliminary study of crystals of the recombinant calcium-regulated photoprotein obelin from the bioluminescent hydroid Obelia longissima

Vysotski, E.S.,Liu, Z.-J.,Rose, J.  et al.
(1999)  Acta Crystallogr.,Sect.D  55 : 1965 - 1966

Structural basis for the emission of violet bioluminescence from a W92F obelin mutant

Deng, L.,Vysotski, E.S.,Liu, Z.-J.  et al.
(2001)  FEBS Lett.  506 : 281 - 285

Violet Bioluminescence and Fast Kinetics from W92F Obelin: Structure-Based Proposals for the Bioluminescence Triggering and the Identification of the Emitting Species

Vysotski, E.S.,Liu, Z.-J.,Markova, S.V.  et al.
(2003)  Biochemistry  42 : 6013 - 6024

Atomic resolution structure of obelin: Soaking with calcium enhances electron density of the second oxygen atom substituted at the C2-position of coelenterazine

Liu, Z.-J.,Vysotski, E.S.,Deng, L.  et al.
(2003)  Biochem.Biophys.Res.Commun.  311 : 433 - 439

Preparation and X-ray Crystallographic Analysis of the Ca2+-discharged Photoprotein Obelin

Deng, L.,Markova, S.V.,Vysotski, E.S.  et al.
To be Published 

Reaction

Chain : A
UniProt : Q27709 (OBL_OBELO)
Reaction : -