Brand  (β version)

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Code Name Link
CO Cobalt (II) ion
OS Osmium ion
Code : 1RMQ
Title : Crystal structure of AphA class B acid phosphatase/phosphotransferase with osmiate mimicking the catalytic intermediate
Release Data : 2004-12-14
Compound :
mol_id molecule chains
1 Class B acid phosphatase A,B
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
gene: APHA, NAPA, B4055, SF4149, S3580
expression_system_strain: DH5alpha
expression_system_vector_type: plasmid
expression_system_plasmid: pATac
Authors : Calderone, V., Forleo, C., Benvenuti, M., Rossolini, G.M., Thaller, M.C., Mangani, S.
Keywords : Class B acid phosphatase, DDDD acid phosphatase, metallo-enzyme, osmiate, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 2.00 Å )
Citation :

Insights in the catalytic mechanism of AphA from Escherichia coli

Calderone, V.,Forleo, C.,Benvenuti, M.  et al.
To be Published 

Identification of the gene (aphA) encoding the class B acid phosphatase/phosphotransferase of Escherichia coli MG1655 and characterization of its product

Thaller, M.C.,Schippa, S.,Bonci, A.  et al.
(1997)  FEMS Microbiol.Lett.  146 : 191 - 198

DOI: 10.1016/S0378-1097(96)00474-0

Expression, purification, crystallization and preliminary X-ray characterization of the class B acid phosphatase (AphA) from Escherichia coli

Forleo, C.,Benvenuti, M.,Calderone, V.  et al.
(2003)  Acta Crystallogr.,Sect.D  59 : 1058 - 1060

DOI: 10.1107/S0907444903006826

Chain : A, B
UniProt : P32697 (multiple entry names are found)
Reaction : -