Brand  (β version)

PDB ID: 1R64

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Ligands
Code Name Style Show Link
CA Calcium ion PoSSuM
K Potassium ion PoSSuM
Non-standard Residues
Code Name Show
BOR (1r)-1-amino-4-{[(E)-amino(imino)methyl]amino}butylboronic acid
Glycosylation
Code Name Emphasize
NAG 2-acetamido-2-deoxy-beta-D-glucopyranose
Modification
Code Name Show
ACE Acetyl group

Structure summary

Code : 1R64   PDBj   RCSB PDB   PDBe
Header : HYDROLASE/HYDROLASE INHIBITOR
Title : The 2.2 A crystal structure of Kex2 protease in complex with Ac-Arg-Glu-Lys-boroArg peptidyl boronic acid inhibitor
Release Data : 2004-03-09
Compound :
mol_id molecule chains synonym
1 Kexin A,B KEX2 protease, Proteinase YSCF
ec: 3.4.21.61
fragment: Residues 121-601
mol_id molecule chains
2 Ac-Arg-Glu-Lys-boroArg peptide inhibitor C,D
Source :
mol_id organism_scientific organism_common expression_system
1 Saccharomyces cerevisiae  (taxid:4932) Baker's yeast Saccharomyces cerevisiae  (taxid:4932)
gene: KEX2 OR QDS1 OR YNL238W OR N1122
expression_system_common: Baker's yeast
expression_system_strain: ASY1
expression_system_vector_type: Plasmid
expression_system_plasmid: PG5
mol_id organism_scientific
2
synthetic: yes
other_details: The peptide was chemically synthesized.
Authors : Holyoak, T., Kettner, C.A., Petsko, G.A., Fuller, R.S., Ringe, D.
Keywords : KEX2, KEXIN, PROTEASE, PROTEIN CONVERTASE, PROHORMONE PROCESSING, PROPROTEIN CONVERTASE, SUBTILISIN, HYDROLASE-HYDROLASE INHIBITOR COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.20 Å )
Citation :

Structural Basis for Differences in Substrate Selectivity in Kex2 and Furin Protein Convertases

Holyoak, T.,Kettner, C.A.,Petsko, G.A.  et al.
(2004)  Biochemistry  43 : 2412 - 2421

PubMed: 14992578
DOI: 10.1021/bi035849h

Reaction

Chain : A, B
UniProt : P13134 (KEX2_YEAST)
Reaction: EC: Evidence:
Physiological Direction:
Cleavage of -Lys-Arg-|-Xaa- and -Arg-Arg-|-Xaa- bonds to process yeast alpha-factor pheromone and killer toxin precursors. 3.4.21.61 -
-