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Ligands
Code Name Style Show Link
Non-standard Residues
Code Name Show
FHU (5s,6r)-5-fluoro-6-hydroxy-pseudouridine-5'-monophosphate
Glycosylation
Code Name Emphasize
Modification
Code Name Show
Code : 1R3E   PDBj   RCSB PDB   PDBe
Header : LYASE/RNA
Title : Crystal Structure of tRNA Pseudouridine Synthase TruB and Its RNA Complex: RNA-protein Recognition Through a Combination of Rigid Docking and Induced Fit
Release Data : 2003-11-04
Compound :
mol_id molecule chains
1 5'-R(*CP*UP*GP*UP*GP*UP*(FHU)P*CP*GP*AP*UP*CP*CP*AP*CP*AP*G)-3' C
mol_id molecule chains
2 5'-R(*CP*UP*GP*UP*GP*UP*UP*CP*GP*AP*UP*CP*CP*AP*CP*AP*G)-3' D,E
mol_id molecule chains synonym
3 tRNA pseudouridine synthase B A tRNA pseudouridine 55 synthase, Psi55 synthase, Pseudouridylate synthase, Uracil hydrolyase
ec: 4.2.1.70
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific expression_system
3 Thermotoga maritima  (taxid:2336) Escherichia coli BL21(DE3)  (taxid:469008)
gene: TRUB
expression_system_strain: BL21DE3
expression_system_vector_type: plasmid
expression_system_plasmid: pET28b
Authors : Pan, H., Agarwalla, S., Moustakas, D.T., Finer-Moore, J., Stroud, R.M.
Keywords : RNA modification, pseudouridylation, LYASE-RNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.1 Å )
Citation :

Crystal Structure of tRNA Pseudouridine Synthase TruB and Its RNA Complex: RNA Recognition Through a Combination of Rigid Docking and Induced Fit

Pan, H.,Agarwalla, S.,Moustakas, D.T.  et al.
(2003)  Proc.Natl.Acad.Sci.USA  100 : 12648 - 12653

PubMed: 14566049
DOI: 10.1073/pnas.2135585100

Chain : A
UniProt : Q9WZW0 (TRUB_THEMA)
Reaction: EC: Evidence:
Physiological Direction:
uridine(55) in tRNA = pseudouridine(55) in tRNA 5.4.99.25 HAMAP-Rule:MF_01080
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