Brand  (β version)

PDB ID: 1QV0

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Ligands
Code Name Style Show Link
CO Cobalt (II) ion PoSSuM
CZH C2-hydroperoxy-coelenterazine PoSSuM
GOL Glycerol PoSSuM
K Potassium ion PoSSuM
Non-standard Residues
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Structure summary

Code : 1QV0   PDBj   RCSB PDB   PDBe
Header : LUMINESCENT PROTEIN
Title : Atomic resolution structure of obelin from Obelia longissima
Release Data : 2003-11-11
Compound :
mol_id molecule chains synonym
1 Obelin A OBL
ec: 1.13.12.5
Source :
mol_id organism_scientific expression_system
1 Obelia longissima  (taxid:32570) Escherichia coli  (taxid:562)
expression_system_strain: BL21-Gold
expression_system_vector_type: plasmid
expression_system_plasmid: pET19-OL8
Authors : Liu, Z.J., Vysotski, E.S., Deng, L., Lee, J., Rose, J., Wang, B.C.
Keywords : Photoprotein, obelin, bioluminescence, atomic resolution, calcium binding, EF-hand, LUMINESCENT PROTEIN
Exp. method : X-RAY DIFFRACTION ( 1.10 Å )
Citation :

Atomic resolution structure of obelin: soaking with calcium enhances electron density of the second oxygen atom substituted at the C2-position of coelenterazine.

Liu, Z.J.,Vysotski, E.S.,Deng, L.  et al.
(2003)  Biochem.Biophys.Res.Commun.  311 : 433 - 439

PubMed: 14592432
DOI: 10.1016/j.bbrc.2003.09.231

Structure of the Ca2+-regulated photoprotein obelin at 1.7 resolution determined directly from its sulfur substructure

Liu, Z.-J.,Vysotski, E.,Chen, C.-J.  et al.
(2000)  Protein Sci.  9 : 2085 - 2093

Preparation and preliminary study of crystals of the recombinant calcium-regulated photoprotein obelin from the bioluminescent hydroid Obelia longissima

Vysotski, E.,Liu, Z.-J.,Rose, J.  et al.
(1999)  Acta Crystallogr.,Sect.D  55 : 1965 - 1966

DOI: 10.1107/S0907444999011828

Structural basis for the emission of violet bioluminescence from a W92F obelin mutant

Deng, L.,Vysotski, E.,Liu, Z.-J.  et al.
(2001)  FEBS Lett.  506 : 281 - 285

DOI: 10.1016/S0014-5793(01)02937-4

Violet Bioluminescence and Fast Kinetics from W92F Obelin: Structure-Based Proposals for the Bioluminescence Triggering and the Identification of the Emitting Species

Vysotski, E.,Liu, Z.-J.,Markova, S.  et al.
(2003)  Biochemistry  42 : 6013 - 6024

DOI: 10.1021/bi027258h

Reaction

Chain : A
UniProt : Q27709 (OBL_OBELO)
Reaction : -