Brand  (β version)

PDB ID: 1QSY

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
DDS 2',3'-dideoxyadenosine triphosphate PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
Code Name Show
2DA 2',3'-dideoxyadenosine-5'-monophosphate
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 1QSY   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/DNA
Title : DDATP-Trapped closed ternary complex of the large fragment of DNA Polymerase I from thermus aquaticus
Release Data : 1999-08-16
Compound :
mol_id molecule chains
1 5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(2DA))-3' B
mol_id molecule chains
2 5'-D(*AP*TP*TP*GP*CP*GP*CP*CP*TP*P*GP*GP*TP*C)-3' C
mol_id molecule chains
3 DNA POLYMERASE I A
ec: 2.7.7.7
fragment: KLENOW FRAGMENT
mutation: A348E
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific expression_system
3 Thermus aquaticus  (taxid:271) Escherichia coli  (taxid:562)
expression_system_vector: PLASMID
expression_system_plasmid: PWB254
Authors : Li, Y., Mitaxov, V., Waksman, G.
Keywords : PROTEIN-DNA COMPLEX, CLOSED, DDATP, POLYMERASE/DNA, TRANSFERASE-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.30 Å )
Citation :

Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation.

Li, Y.,Mitaxov, V.,Waksman, G.
(1999)  Proc.Natl.Acad.Sci.USA  96 : 9491 - 9496

PubMed: 10449720
DOI: 10.1073/pnas.96.17.9491

Reaction

Chain : A
UniProt : P19821 (DPO1_THEAQ)
Reaction: EC: Evidence:
Physiological Direction:
a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 2.7.7.7 -
-