Brand  (β version)

PDB ID: 1QSL

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Ligands
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EU Europium ion PoSSuM
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Glycosylation
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Structure summary

Code : 1QSL   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/DNA
Title : KLENOW FRAGMENT COMPLEXED WITH SINGLE-STRANDED SUBSTRATE AND EUROPIUM (III) ION
Release Data : 1999-06-30
Compound :
mol_id molecule chains
1 5'-D(*GP*CP*TP*TP*AP*CP*GP*C)-3' B
mol_id molecule chains synonym
2 DNA POLYMERASE I A POL I
ec: 2.7.7.7
fragment: KLENOW FRAGMENT
mutation: V324M
Source :
mol_id organism_scientific
1
synthetic: yes
other_details: CHEMICALLY SYNTHESIZED BY STANDARD PHOSPHORAMIDITE METHODOLOGY
mol_id organism_scientific expression_system
2 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
expression_system_strain: CJ376
expression_system_vector_type: PLASMID
expression_system_plasmid: PCJ155
Authors : Brautigam, C.A., Aschheim, K., Steitz, T.A.
Keywords : EXONUCLEASE, TWO METAL IONS, SINGLE-STRANDED DNA, TRANSFERASE/DNA, TRANSFERASE-DNA complex
Exp. method : X-RAY DIFFRACTION ( 2.20 Å )
Citation :

Structural elucidation of the binding and inhibitory properties of lanthanide (III) ions at the 3'-5' exonucleolytic active site of the Klenow fragment

Brautigam, C.A.,Aschheim, K.,Steitz, T.A.
(1999)  Chem.Biol.  6 : 901 - 908

PubMed: 10631518
DOI: 10.1016/S1074-5521(00)80009-5

Structural Principles for the Inhibition of the 3'-5' Exonuclease Activity of Escherichia Coli DNA Polymerase I by Phosphorothioates

Brautigam, C.A.,Steitz, T.A.
(1998)  J.Mol.Biol.  277 : 363 - 377

DOI: 10.1006/jmbi.1997.1586

Structural Basis for the 3'-5' Exonuclease Activity of Escherichia Coli DNA Polymerase I: A Two Metal Ion Mechanism

Beese, L.S.,Steitz, T.A.
(1991)  Embo J.  10 : 25 - 33

Reaction

Chain : A
UniProt : P00582 (DPO1_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 2.7.7.7 -
-