Brand  (β version)

PDB ID: 1Q9X

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Code Name Style Show Link
3DR 1',2'-dideoxyribofuranose-5'-phosphate PoSSuM
DOC 2',3'-dideoxycytidine-5'-monophosphate PoSSuM
CA Calcium ion PoSSuM

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Structure summary

Code : 1Q9X   PDBj   RCSB PDB   PDBe
Header : Transferase,replication/DNA
Title : Crystal structure of Enterobacteria phage RB69 gp43 DNA polymerase complexed with tetrahydrofuran containing DNA
Release Data : 2004-04-27
Compound :
mol_id molecule chains
1 5'-GCGGACTGCTTAC(dideoxycytidine)-3' E,F,G,H
mol_id molecule chains
2 5'-AC(tetrahydrofuran)GGTAAGCAGTCCGCGG-3' I,J,K,L
mol_id molecule chains synonym
3 DNA polymerase A,B,C,D Gp43
ec: 2.7.7.7
mutation: D222A, D327A
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific expression_system
3 Enterobacteria phage RB69  (taxid:12353) Escherichia coli  (taxid:562)
gene: 43
expression_system_strain: M15
expression_system_vector_type: plasmid
expression_system_plasmid: pQE30
Authors : Freisinger, E., Grollman, A.P., Miller, H., Kisker, C.
Keywords : PROTEIN-DNA COMPLEX, Transferase, replication-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.69 Å )
Citation :

Lesion (in)tolerance reveals insights into DNA replication fidelity.

Freisinger, E.,Grollman, A.P.,Miller, H.  et al.
(2004)  Embo J.  23 : 1494 - 1505

PubMed: 15057282
DOI: 10.1038/sj.emboj.7600158

Reaction

Chain : A, B, C, D
UniProt : Q38087 (DPOL_BPR69)
Reaction: EC: Evidence:
Physiological Direction:
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 2.7.7.7 HAMAP-Rule:MF_04100, PubMed:17098747, PubMed:18503083, PubMed:20795733, PubMed:22616982, PubMed:24116139
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Cofactor: Evidence: Note:
Mg(2+) ECO:0000255 | HAMAP-Rule:MF_04100
ECO:0000269 | PubMed:20166752
ECO:0000269 | PubMed:22026756
ECO:0000305 | PubMed:11389835
ECO:0000305 | PubMed:15057282
ECO:0000305 | PubMed:15057283
ECO:0000305 | PubMed:17718515
ECO:0000305 | PubMed:18503083
ECO:0000305 | PubMed:20166748
ECO:0000305 | PubMed:21158418
ECO:0000305 | PubMed:21566148
ECO:0000305 | PubMed:21923197
ECO:0000305 | PubMed:22571765
ECO:0000305 | PubMed:23214497
ECO:0000305 | PubMed:23921641
ECO:0000305 | PubMed:24116139
-