Brand  (β version)

PDB ID: 1Q2R

Hetero Atom Contents

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
ZN Zinc ion PoSSuM
9DG 9-deazaguanine PoSSuM
Modified Residues
Code Name Link
N Any 5'-monophosphate nucleotide PoSSuM

Structure summary

Code : 1Q2R
Header : Transferase/RNA
Title : Chemical trapping and crystal structure of a catalytic tRNA guanine transglycosylase covalent intermediate
Release Data : 2003-09-09
Compound :
mol_id molecule chains
1 RNA (5'-R(*AP*GP*CP*AP*CP*GP*GP*CP*UP*(N)P*UP*AP*AP*AP*CP*CP*GP*UP*GP*C)-3') E,F
mol_id molecule chains synonym
2 Queuine tRNA-ribosyltransferase A,B,C,D tRNA-guanine transglycosylase, Guanine insertion enzyme
ec: 2.4.2.29
Source :
mol_id organism_scientific
1 Synthetic construct  (taxid:32630)
synthetic: yes
mol_id organism_scientific expression_system
2 Zymomonas mobilis  (taxid:542) Escherichia coli  (taxid:562)
strain: ZM4-CP4
atcc: 31821
gene: TGT
Authors : Xie, W., Liu, X., Huang, R.H.
Keywords : TIM barrel, Protein-RNA complex, Covalent intermediate, Transferase-RNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.9 Å )
Citation :

Chemical trapping and crystal structure of a catalytic tRNA guanine transglycosylase covalent intermediate

Xie, W.,Liu, X.,Huang, R.H.
(2003)  Nat.Struct.Biol.  10 : 781 - 788

PubMed: 12949492
DOI: 10.1038/nsb976

Reaction

Chain : A, B, C, D
UniProt : P28720 (TGT_ZYMMO)
Reaction : Guanine(34) in tRNA + 7-aminomethyl-7- carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.