Brand  (β version)

PDB ID: 1PXV

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Ligands
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SO4 Sulfate ion PoSSuM
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Structure summary

Code : 1PXV   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : The staphostatin-staphopain complex: a forward binding inhibitor in complex with its target cysteine protease
Release Data : 2003-10-21
Compound :
mol_id molecule chains
1 cysteine protease A,B
ec: 3.4.22.-
mutation: C243A
mol_id molecule chains
2 cysteine protease Inhibitor C,D
ec: 3.4.22.-
Source :
mol_id organism_scientific expression_system
1 Staphylococcus aureus  (taxid:1280) Escherichia coli  (taxid:562)
gene: staphopain B
expression_system_strain: E. coli BL21 (DE3)
expression_system_vector_type: pET15
mol_id organism_scientific expression_system
2 Staphylococcus aureus  (taxid:1280) Escherichia coli  (taxid:562)
gene: staphostatin B
expression_system_strain: E. coli BL21 (DE3)[pLysS]
expression_system_vector_type: pGEX-5T
Authors : Filipek, R., Rzychon, M., Oleksy, A., Gruca, M., Dubin, A., Potempa, J., Bochtler, M.
Keywords : cysteine protease inhibitor, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 1.80 Å )
Citation :

The Staphostatin-Staphopain Complex: A FORWARD BINDING INHIBITOR IN COMPLEX WITH ITS TARGET CYSTEINE PROTEASE.

Filipek, R.,Rzychon, M.,Oleksy, A.  et al.
(2003)  J.Biol.Chem.  278 : 40959 - 40966

PubMed: 12874290
DOI: 10.1074/jbc.M302926200

Staphostatins resemble lipocalins, not cystatins in fold.

Rzychon, M.,Filipek, R.,Sabat, A.  et al.
(2003)  Protein Sci.  12 : 2252 - 2256

DOI: 10.1110/ps.03247703

Staphostatins: an expanding new group of proteinase inhibitors with a unique specificity for the regulation of staphopains, Staphylococcus spp. cysteine proteinases

Rzychon, M.,Sabat, A.,Kosowska, K.  et al.
(2003)  MOL.MICROBIOL.  49 : 1051 - 1066

DOI: 10.1046/j.1365-2958.2003.03613.x

Identification of a novel maturation mechanism and restricted substrate specificity for the SspB cysteine protease of Staphylococcus aureus

Massimi, I.,Park, E.,Rice, K.  et al.
(2002)  J.Biol.Chem.  277 : 41770 - 41777

DOI: 10.1074/jbc.M207162200

Reaction

Chain : A, B
UniProt : Q70UQ8
Reaction : -
Chain : C, D
UniProt : Q9EYW6 (SSPC_STAA8)
Reaction : -