Brand  (β version)

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Modified Residues
Code Name Link
T Thymidine-5'-monophosphate
Code : 1PVP
Header : RECOMBINATION/DNA
Title : BASIS FOR A SWITCH IN SUBSTRATE SPECIFICITY: CRYSTAL STRUCTURE OF SELECTED VARIANT OF CRE SITE-SPECIFIC RECOMBINASE, ALSHG BOUND TO THE ENGINEERED RECOGNITION SITE LOXM7
Release Data : 2004-02-17
Compound :
mol_id molecule chains
1 34-MER C
other_details: TOP STRAND OF LOXM7 ENGINEERED DNA SUBSTRATE (LOXP(C7/G28,T8/A27,A9/T26)
mol_id molecule chains
2 34-MER D
other_details: BOTTOM STRAND OF LOXM7 ENGINEERED DNA SUBSTRATE (LOXP(C7/G28,T8/A27,A9/T26)
mol_id molecule chains
3 Recombinase CRE A,B
mutation: I174A,T258L,R259S,E262H,E266G
other_details: Cre site-specific recombinase mutant (I174A, T258L, R259S, E262H, E266G)
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific expression_system
3 Enterobacteria phage P1  (taxid:10678) Escherichia coli BL21(DE3)  (taxid:469008)
gene: CRE
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: PET28B(+)
Authors : Baldwin, E.P., Martin, S.S., Abel, J., Gelato, K.A., Kim, H., Schultz, P.G., Santoro, S.W.
Keywords : Cre, recombinase, DNA, CRE-LOXP RECOMBINATION, RECOMBINATION-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.35 Å )
Citation :

A specificity switch in selected cre recombinase variants is mediated by macromolecular plasticity and water.

Baldwin, E.P.,Martin, S.S.,Abel, J.  et al.
(2003)  Chem.Biol.  10 : 1085 - 1094

PubMed: 14652076
DOI: 10.1016/j.chembiol.2003.10.015

Directed evolution of the site specificity of Cre recombinase.

Santoro, S.W.,Schultz, P.G.
(2002)  Proc.Natl.Acad.Sci.USA  99 : 4185 - 4190

DOI: 10.1073/pnas.022039799

Modulation of the active complex assembly and turnover rate by protein-DNA interactions in Cre-LoxP recombination.

Martin, S.S.,Chu, V.C.,Baldwin, E.
(2003)  Biochemistry  42 : 6814 - 6826

DOI: 10.1021/bi0272306

The order of strand exchanges in Cre-LoxP recombination and its basis suggested by the crystal structure of a Cre-LoxP Holliday junction complex.

Martin, S.S.,Pulido, E.,Chu, V.C.  et al.
(2002)  J.Mol.Biol.  319 : 107 - 127

DOI: 10.1016/S0022-2836(02)00246-2

Chain : A, B
UniProt : P06956 (RECR_BPP1)
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