Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
FAS Arabino-flavin-adenine dinucleotide
PHB P-hydroxybenzoic acid
Code : 1PDH
Header : OXIDOREDUCTASE
Title : CRYSTAL STRUCTURE OF P-HYDROXYBENZOATE HYDROXYLASE RECONSTITUTED WITH THE MODIFIED FAD PRESENT IN ALCOHOL OXIDASE FROM METHYLOTROPHIC YEASTS: EVIDENCE FOR AN ARABINOFLAVIN
Release Data : 1995-03-31
Compound :
mol_id molecule chains
1 P-HYDROXYBENZOATE HYDROXYLASE A
ec: 1.14.13.2
Source :
mol_id organism_scientific
1 Pseudomonas fluorescens  (taxid:294)
Authors : Schreuder, H.A., Eppink, M.H.M., Van Berkel, W.J.H.
Keywords : OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 2.1 Å )
Citation :

Crystal structure of p-hydroxybenzoate hydroxylase reconstituted with the modified FAD present in alcohol oxidase from methylotrophic yeasts: evidence for an arabinoflavin.

van Berkel, W.J.,Eppink, M.H.,Schreuder, H.A.
(1994)  Protein Sci.  3 : 2245 - 2253

PubMed: 7756982

Crystal Structure of Wild-Type P-Hydroxybenzoate Hydroxylase Complexed with 4-Aminobenzoate, 2,4-Dihydroxybenzoate and 2-Hydroxy-4-Aminobenzoate. Evidence for a Proton Channel and a New Binding Mode of the Flavin Ring

Schreuder, H.A.,Mattevi, A.,Obmolova, G.  et al.
(1994)  Biochemistry  33 : 10161

Crystal Structure of the Reduced Form of P-Hydroxybenzoate Hydroxylase Refine at 2.3 Angstroms Resolution

Schreuder, H.A.,Van Der Laan, J.M.,Swarte, M.B.A.  et al.
(1992)  Proteins  14 : 178

Crystal Structure of the P-Hydroxybenzoate Hydroxylase-Substrate Complex Refined at 1.9 Angstroms Resolution

Schreuder, H.A.,Prick, P.A.J.,Wierenga, R.K.  et al.
(1989)  J.Mol.Biol.  208 : 679

Analysis of the Active Site of the Flavoprotein P-Hydroxybenzoate Hydroxylase and Some Ideas with Respect to its Reaction Mechanism

Schreuder, H.A.,Hol, W.G.J.,Drenth, J.
(1989)  Biochemistry  29 : 3101

The Coenzyme Analogue Adenosine 5-Diphosphoribose Displaces the Fad from the Active Site of P-Hydroxybenzoate Hydroxylase. An X-Ray Crystallographic Investigation

Van Der Laan, J.M.,Schreuder, H.A.,Swarte, M.B.A.  et al.
(1989)  Biochemistry  28 : 7199

The Influence of Purification and Protein Heterogeneity on the Crystallization of P-Hydroxybenzoate Hydroxylase

Van Der Laan, J.M.,Swarte, M.B.A.,Groendijk, H.  et al.
(1989)  Eur.J.Biochem.  179 : 715

Crystal Structure of P-Hydroxybenzoate Hydroxylase Complexed with its Reaction Product 3,4-Dihydroxybenzoate

Schreuder, H.A.,Van Der Laan, J.M.,Hol, W.G.J.  et al.
(1988)  J.Mol.Biol.  199 : 637

Molecular Modeling Reveals the Possible Importance of a Carbonyl Oxygen Binding Pocket for the Catalytic Mechanism of P-Hydroxybenzoate Hydroxylase

Schreuder, H.A.,Hol, W.G.J.,Drenth, J.
(1988)  J.Biol.Chem.  263 : 3131

Comparison of the Three-Dimensional Protein and Nucleotide Structure of the Fad-Binding Domain of P-Hydroxybenzoate Hydroxylase with the Fad-as Well as Nadph-Binding Domains of Glutathione Reductase

Wierenga, R.K.,Drenth, J.,Schultz, G.E.
(1983)  J.Mol.Biol.  167 : 725

Crystal Structure of P-Hydroxybenzoate Hydroxylase

Wierenga, R.K.,De Jong, R.J.,Kalk, K.H.  et al.
(1979)  J.Mol.Biol.  131 : 55

Crystallization and Preliminary X-Ray Investigation of P-Hydroxybenzoate Hydroxylase from Pseudomonas Fluorescens

Drenth, J.,Hol, W.G.J.,Wierenga, R.K.
(1975)  J.Biol.Chem.  250 : 5268

Chain : A
UniProt : P00438 (PHHY_PSEFL)
Reaction : 4-hydroxybenzoate + NADPH + O(2) = protocatechuate + NADP(+) + H(2)O.