Brand  (β version)

color scheme of protein:

hetatm:

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information
centroid:
interaction residue:

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Ligands
Code Name Link
CA Calcium ion
NAG 2-acetamido-2-deoxy-beta-D-glucopyranose
SO4 Sulfate ion
Modified Residues
Code Name Link
0QE Chloromethane
AR7 Amino{[(4s)-4-amino-5,5-dihydroxypentyl]amino}methaniminium
BMA Beta-D-mannopyranose
DKA Decanoic acid
FUC Alpha-L-fucopyranose
FUL Beta-L-fucopyranose
GAL Beta-D-galactopyranose
MAN Alpha-D-mannopyranose
Code : 1P8J
Header : HYDROLASE/HYDROLASE INHIBITOR
Title : CRYSTAL STRUCTURE OF THE PROPROTEIN CONVERTASE FURIN
Release Data : 2003-07-08
Compound :
mol_id molecule chains synonym
1 Furin precursor A,B,C,D,E,F,G,H Paired basic amino acid residue cleaving enzyme, PACE, Dibasic processing enzyme
ec: 3.4.21.75
mol_id molecule chains
2 DECANOYL-ARG-VAL-LYS-ARG-CHLOROMETHYLKETONE INHIBITOR J,K,L,M,N,P,Q,R
Source :
mol_id organism_scientific organism_common expression_system
1 Mus musculus  (taxid:10090) House mouse Cricetulus griseus  (taxid:10029)
gene: FURIN OR FUR OR PCSK3
expression_system_common: Chinese hamster
expression_system_strain: DG44
expression_system_organ: Ovary cells
expression_system_vector_type: Plasmid
expression_system_plasmid: pcDNA3.1(-)-Myc-His
mol_id organism_scientific
2
synthetic: yes
other_details: THe peptide (decanoyl-Arg-Val-Lys-Arg-chloromethylketone) was chemically synthesized
Authors : Henrich, S., Cameron, A., Bourenkov, G.P., Kiefersauer, R., Huber, R., Lindberg, I., Bode, W., Than, M.E.
Keywords : PROHORMONE CONVERTASE, SPC1, PACE, P-DOMAIN, CHLOROMETHYLKETONE, HYDROLASE-HYDROLASE INHIBITOR COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.60 Å )
Citation :

The Crystal Structure of the Proprotein Processing Proteinase Furin Explains its Stringent Specificity

Henrich, S.,Cameron, A.,Bourenkov, G.P.  et al.
(2003)  Nat.Struct.Biol.  10 : 520 - 526

PubMed: 12794637
DOI: 10.1038/nsb941

Chain : A, B, C, D, E, F, G, H
UniProt : P23188 (FURIN_MOUSE)
Reaction : Release of mature proteins from their proproteins by cleavage of -Arg-Xaa-Yaa-Arg-|-Zaa- bonds, where Xaa can be any amino acid and Yaa is Arg or Lys. Releases albumin, complement component C3 and von Willebrand factor from their respective precursors.