Brand  (β version)

PDB ID: 1P16

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Ligands
Code Name Style Show Link
PO4 Phosphate ion PoSSuM
5GP Guanosine-5'-monophosphate PoSSuM
Non-standard Residues
Code Name Show
MSE Selenomethionine
SEP Phosphoserine
Glycosylation
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Structure summary

Code : 1P16   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Structure of an mRNA capping enzyme bound to the phosphorylated carboxyl-terminal domain of RNA polymerase II
Release Data : 2003-07-15
Compound :
mol_id molecule chains synonym
1 mRNA capping enzyme alpha subunit A,B mRNA guanylyltransferase; GTP--RNA guanylyltransferase; GTase
ec: 2.7.7.50
fragment: residues 1-395 od SWS P78587
mutation: I2V
mol_id molecule chains synonym
2 phosphorylated peptide from C-terminal of RNA polymerase II C,D phosphorylated CTD peptide
Source :
mol_id organism_scientific expression_system
1 Candida albicans  (taxid:5476) Escherichia coli  (taxid:562)
gene: CGT1
expression_system_variant: B834 (DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pSMT3
other_details: modified pet15b (N-terminal SUMO fusion)
mol_id organism_scientific
2
synthetic: yes
other_details: NATURALLY OCCURING REPETITIVE YSPTSPS SEQUENCE
Authors : Fabrega, C., Shen, V., Shuman, S., Lima, C.D.
Keywords : GUANYLYLTRANSFERASE, CANDIDA ALBICANS, RNA POLYMERASE II, TRANSCRIPTION, CAPPING, CTD, MRNA, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.70 Å )
Citation :

Structure of an mRNA capping enzyme bound to the phosphorylated carboxy-terminal domain of RNA polymerase II.

Fabrega, C.,Shen, V.,Shuman, S.  et al.
(2003)  Mol.Cell  11 : 1549 - 1561

PubMed: 12820968
DOI: 10.1016/S1097-2765(03)00187-4

Reaction

Chain : A, B
UniProt : P78587 (MCE1_CANAX)
Reaction: EC: Evidence:
Physiological Direction:
a 5'-end diphospho-ribonucleoside in mRNA + GTP + H(+) = a 5'- end (5'-triphosphoguanosine)-ribonucleoside in mRNA + diphosphate 2.7.7.50 UniProtKB:Q01159
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