Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
0E8 Di-tert-butyl {iminobis[(2s,3s)-3-hydroxy-1-phenylbutane-4,2-diyl]}biscarbamate
Code : 1ODW   PDBj   RCSB PDB   PDBe
Header : Hydrolase/Hydrolase Inhibitor
Title : Native HIV-1 Proteinase
Release Data : 1997-04-01
Compound :
mol_id molecule chains synonym
1 HIV-1 PROTEASE A,B RETROPEPSIN
ec: 3.4.23.16
Source :
mol_id organism_scientific organism_common expression_system
1 Human immunodeficiency virus 1  (taxid:11676) HIV-1 Escherichia coli  (taxid:562)
gene: gag-pol
expression_system_plasmid: PMAL-C2
Authors : Thanki, N., Kervinen, J., Wlodawer, A.
Keywords : PROTEINASE, RETROPEPSIN, RETROVIRUS, HIV, Hydrolase-Hydrolase Inhibitor complex
Exp. method : X-RAY DIFFRACTION ( 2.1 Å )
Citation :

Structural Analysis of the Native and Drug-Resistant HIV-1 Proteinases Complexed with an Aminodiol Inhibitor

Kervinen, J.,Thanki, N.,Zdanov, A.  et al.
(1996)  Protein Pept.Lett.  3 : 399

PubMed: -1

Chain : A, B
UniProt : P04585 (POL_HV1H2)
Reaction : Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).