Het-PDB Navi2 (PDB: 1NQJ)

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Ligands
Code	Name		Style	Show	Link
CL	Chloride ion				PoSSuM
LI	Lithium ion				PoSSuM

Non-standard Residues
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Glycosylation
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Download interaction data: 1NQJ

Code :

1NQJ PDBj RCSB PDB PDBe

Header :

HYDROLASE

Title :

CRYSTAL STRUCTURE OF CLOSTRIDIUM HISTOLYTICUM COLG COLLAGENASE COLLAGEN-BINDING DOMAIN 3B AT 1.0 ANGSTROM RESOLUTION IN ABSENCE OF CALCIUM

Release Data :

2003-04-15

Compound :

mol_id	molecule	chains
1	class 1 collagenase	A,B
	ec: 3.4.24.3 fragment: COLLAGEN-BINDING DOMAIN

Source :

mol_id	organism_scientific	expression_system
1	Clostridium histolyticum (taxid:1498)	Escherichia coli BL21 (taxid:511693)
	gene: ColG expression_system_strain: BL21 expression_system_vector_type: PLASMID expression_system_plasmid: pGEX-4T-2

Authors :

Wilson, J.J., Matsushita, O., Okabe, A., Sakon, J.

Keywords :

BETA SANDWICH, METALLOPROTEASE, COLLAGEN-BINDING DOMAIN, LITHIUM, CHLORINE, HYDROLASE

Exp. method :

X-RAY DIFFRACTION ( 1.00 Å )

Citation :

A bacterial collagen-binding domain with novel calcium-binding motif controls domain orientation

Wilson, J.J.,Matsushita, O.,Okabe, A. et al.
(2003) Embo J. 22 : 1743 - 1752

Chain :

A, B

UniProt :

Q9S0X0 (COLG_HATHI)

Reaction:	EC:	Evidence:
Reaction:	Physiological Direction:	Evidence:
Digestion of native collagen in the triple helical region at Xaa-\|-Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1', Pro and Ala at P2 and P2', and hydroxyproline, Ala or Arg at P3'.	3.4.24.3	PubMed:24125730, PubMed:3002446, PubMed:18937627
	-	PubMed:24125730, PubMed:3002446, PubMed:18937627
Cofactor:	Evidence:	Note:
Ca(2+)	ECO:0000269 \| PubMed:12682007 ECO:0000269 \| PubMed:23144249 ECO:0000269 \| PubMed:23703618 ECO:0000269 \| PubMed:6087888 ECO:0000269 \| Ref.21 ECO:0000269 \| Ref.27	Binds about 7 Ca(2+) per subunit (PubMed:6087888). The metallopeptidase and PKD domains each bind 1 Ca(2+), while each CDB binds 2 (PubMed:12682007, PubMed:23144249, Ref.21, Ref.27).
Zn(2+)	ECO:0000269 \| PubMed:21947205 ECO:0000269 \| PubMed:23703618 ECO:0000269 \| PubMed:6087888	Binds 1 catalytic Zn(2+) per subunit, a Zn-free form has been crystallized (PubMed:21947205).

PDB ID: 1NQJ