Brand  (β version)

PDB ID: 1NJY

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Ligands
Code Name Style Show Link
MG Magnesium ion PoSSuM
SO4 Sulfate ion PoSSuM
TTP Thymidine-5'-triphosphate PoSSuM
FRU Beta-D-fructofuranose PoSSuM
GLC Alpha-D-glucopyranose PoSSuM
: Polysaccharide
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Structure summary

Code : 1NJY   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/DNA
Title : THYMINE-THYMINE MISMATCH AT THE POLYMERASE ACTIVE SITE
Release Data : 2004-03-30
Compound :
mol_id molecule chains
1 DNA PRIMER STRAND B
mol_id molecule chains
2 DNA TEMPLATE STRAND C
mol_id molecule chains
3 DNA POLYMERASE I A
ec: 2.7.7.7
fragment: BACILLUS FRAGMENT (ANALOGOUS TO THE E. COLI KLENOW FRAGMENT)
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific expression_system
3 Geobacillus stearothermophilus  (taxid:1422) Escherichia coli  (taxid:562)
expression_system_strain: BL21(DE3)PLYSS
expression_system_vector_type: PLASMID
expression_system_plasmid: PET-30A(+)
Authors : Johnson, S.J., Beese, L.S.
Keywords : DNA POLYMERASE I, DNA REPLICATION, KLENOW FRAGMENT, PROTEIN-DNA COMPLEX, DNA MISMATCH, TRANSFERASE-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.00 Å )
Citation :

Structures of mismatch replication errors observed in a DNA polymerase.

Johnson, S.J.,Beese, L.S.
(2004)  Cell(Cambridge,Mass.)  116 : 803 - 816

PubMed: 15035983
DOI: 10.1016/S0092-8674(04)00252-1

Processive DNA synthesis observed in a polymerase crystal suggests a mechanism for the prevention of frameshift mutations.

Johnson, S.J.,Taylor, J.S.,Beese, L.S.
(2003)  Proc.Natl.Acad.Sci.USA  100 : 3895 - 3900

PubMed: 12649320
DOI: 10.1073/pnas.0630532100

Visualizing DNA Replication in a Catalytically Active Bacillus DNA Polymerase Crystal

Kiefer, J.R.,Mao, C.,Braman, J.C.  et al.
(1998)  Nature  391 : 304 - 307

DOI: 10.1038/34693

Crystal Structure of a Thermostable Bacillus DNA Polymerase I Large Fragment at 2.1 A Resolution

Kiefer, J.R.,Mao, C.,Hansen, C.J.  et al.
(1997)  Structure  5 : 95 - 108

DOI: 10.1016/S0969-2126(97)00169-X

Reaction

Chain : A
UniProt : P52026 (DPO1_GEOSE)
Reaction: EC: Evidence:
Physiological Direction:
a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 2.7.7.7 -
-