Brand  (β version)

PDB ID: 1MA0

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Ligands
Code Name Style Show Link
DAO Lauric acid PoSSuM
K Potassium ion PoSSuM
NAD Nicotinamide-adenine-dinucleotide PoSSuM
PO4 Phosphate ion PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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Structure summary

Code : 1MA0   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Ternary complex of Human glutathione-dependent formaldehyde dehydrogenase with NAD+ and dodecanoic acid
Release Data : 2002-08-02
Compound :
mol_id molecule chains synonym
1 Glutathione-dependent formaldehyde dehydrogenase A,B alcohol dehydrogenase class III chi chain; Alcohol dehydrogenase (class III), chi polypeptide; FDH; E.C.1.2.1.1
ec: 1.1.1.1
other_details: Apoenzyme
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: ADH5
expression_system_strain: TG-1
expression_system_vector_type: PLASMID
expression_system_plasmid: pKK223-3
Authors : Sanghani, P.C., Robinson, H., Bosron, W.F., Hurley, T.D.
Keywords : human glutathione-dependent formaldehyde dehydrogenase, class III alcohol dehydrogenase, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 2.30 Å )
Citation :

Human glutathione-dependent formaldehyde dehydrogenase. Structures of apo, binary, and inhibitory ternary complexes.

Sanghani, P.C.,Robinson, H.,Bosron, W.F.  et al.
(2002)  Biochemistry  41 : 10778 - 10786

PubMed: 12196016
DOI: 10.1021/bi0257639

Reaction

Chain : A, B
UniProt : P11766 (ADHX_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
a primary alcohol + NAD(+) = an aldehyde + H(+) + NADH 1.1.1.1 PubMed:8460164
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a secondary alcohol + NAD(+) = a ketone + H(+) + NADH 1.1.1.1 PubMed:8460164
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NADP(+) + S-(hydroxymethyl)glutathione = H(+) + NADPH + S- formylglutathione 1.1.1.284 PubMed:8460164
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NAD(+) + S-(hydroxymethyl)glutathione = H(+) + NADH + S- formylglutathione 1.1.1.284 PubMed:8460164
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20-oxo-(5Z,8Z,11Z,14Z)-eicosatetraenoate + H2O + NAD(+) = (5Z,8Z,11Z,14Z)-eicosatetraenedioate + 2 H(+) + NADH - PubMed:16081420
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20-hydroxy-(5Z,8Z,11Z,14Z)-eicosatetraenoate + NAD(+) = 20- oxo-(5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+) + NADH - PubMed:16081420
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