Brand  (β version)

PDB ID: 1M75

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Ligands
Code Name Style Show Link
CAA Acetoacetyl-coenzyme a PoSSuM
NAD Nicotinamide-adenine-dinucleotide PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1M75   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Crystal Structure of the N208S Mutant of L-3-Hydroxyacyl-COA Dehydrogenase in Complex with NAD and Acetoacetyl-COA
Release Data : 2004-02-03
Compound :
mol_id molecule chains synonym
1 3-HYDROXYACYL-COA DEHYDROGENASE A,B L-3-HYDROXYACYL-COA DEHYDROGENASE
ec: 1.1.1.35
fragment: SHORT CHAIN
mutation: N208S
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: HCDH
expression_system_strain: BL21(DE3)PLYSS
expression_system_vector_type: PLASMID
expression_system_plasmid: pET28a
Authors : Barycki, J.J., Banaszak, L.J.
Keywords : ROSSMANN FOLD, DEHYDROGENASE, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 2.30 Å )
Citation :

Crystal Structure of the N208S Mutant of L-3-Hydroxyacyl-COA Dehydrogenase in Complex with NAD and Acetoacetyl-COA

Barycki, J.J.,Banaszak, L.J.
To be Published 

Biochemical characterization and crystal structure determination of human heart short chain L-3-hydroxyacyl-COA dehydrogenase provide insights into catalytic mechanism

Barycki, J.J.,O'Brien, L.K.,Bratt, J.M.  et al.
(1999)  Biochemistry  38 : 5786 - 5798

DOI: 10.1021/bi9829027

Reaction

Chain : A, B
UniProt : Q16836 (HCDH_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) + NADH 1.1.1.35 PubMed:10231530, PubMed:11489939, PubMed:16725361
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(3S)-3-hydroxybutanoyl-CoA + NAD(+) = acetoacetyl-CoA + H(+) + NADH - PubMed:10231530, PubMed:16725361
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(3S)-hydroxydecanoyl-CoA + NAD(+) = 3-oxodecanoyl-CoA + H(+) + NADH - UniProtKB:P00348
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(3S)-hydroxyhexadecanoyl-CoA + NAD(+) = 3-oxohexadecanoyl-CoA + H(+) + NADH - UniProtKB:P00348
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