Brand  (β version)

PDB ID: 1M6V

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Ligands
Code Name Style Show Link
ADP Adenosine-5'-diphosphate PoSSuM
CL Chloride ion PoSSuM
K Potassium ion PoSSuM
MN Manganese (II) ion PoSSuM
NET Tetraethylammonium ion PoSSuM
ORN L-ornithine PoSSuM
PO4 Phosphate ion PoSSuM
Non-standard Residues
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Structure summary

Code : 1M6V   PDBj   RCSB PDB   PDBe
Header : LIGASE
Title : Crystal Structure of the G359F (small subunit) Point Mutant of Carbamoyl Phosphate Synthetase
Release Data : 2002-07-31
Compound :
mol_id molecule chains synonym
1 carbamoyl phosphate synthetase large chain A,C,E,G Carbamoyl-phosphate synthetase ammonia chain
ec: 6.3.5.5
mol_id molecule chains synonym
2 carbamoyl-phosphate synthetase small chain B,D,F,H Carbamoyl-phosphate synthetase glutamine chain
ec: 6.3.5.5
mutation: G359F
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
gene: CARB
expression_system_strain: BL21(de3)pLysS
expression_system_vector_type: plasmid
mol_id organism_scientific expression_system
2 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
gene: CARA
expression_system_strain: BL21(de3)pLysS
expression_system_vector_type: plasmid
Authors : Thoden, J.B., Huang, X., Raushel, F.M., Holden, H.M.
Keywords : substrate channeling, tunnel, LIGASE
Exp. method : X-RAY DIFFRACTION ( 2.10 Å )
Citation :

Carbamoyl-phosphate synthetase. Creation of an escape route for ammonia

Thoden, J.B.,Huang, X.,Raushel, F.M.  et al.
(2002)  J.Biol.Chem.  277 : 39722 - 39727

PubMed: 12130656
DOI: 10.1074/jbc.M206915200

Reaction

Chain : A, C, E, G
UniProt : P00968 (CARB_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate 6.3.5.5 HAMAP- Rule:MF_01210, PubMed:4944634
-
[Carbamoyl phosphate synthase large chain]
2 ATP + hydrogencarbonate + NH4(+) = 2 ADP + carbamoyl phosphate + 2 H(+) + phosphate 		6.3.4.16 HAMAP- Rule:MF_01210, PubMed:4944634
-
Chain : B, D, F, H
UniProt : P00907 (CARA_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate 6.3.5.5 HAMAP- Rule:MF_01209, PubMed:4944634
-
[Carbamoyl phosphate synthase small chain]
H2O + L-glutamine = L-glutamate + NH4(+) 		- HAMAP- Rule:MF_01209, PubMed:4944634
-