Brand  (β version)

PDB ID: 1LV5

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
MN Manganese (II) ion PoSSuM
MG Magnesium ion PoSSuM
SO4 Sulfate ion PoSSuM
DCP 2'-deoxycytidine-5'-triphosphate PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 1LV5   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/DNA
Title : Crystal Structure of the Closed Conformation of Bacillus DNA Polymerase I Fragment Bound to DNA and dCTP
Release Data : 2003-03-25
Compound :
mol_id molecule chains
1 5'-D(*GP*GP*AP*TP*CP*AP*GP*CP*GP*A)-3' C,E
other_details: PRIMER STRAND
mol_id molecule chains
2 5'-D(*AP*CP*GP*TP*CP*GP*CP*TP*GP*AP*TP*CP*CP*G)-3' D,F
other_details: Template Strand
mol_id molecule chains
3 DNA POLYMERASE I A,B
ec: 2.7.7.7
fragment: BACILLUS FRAGMENT (ANALOGOUS TO THE E. COLI KLENOW FRAGMENT)
mutation: D329A
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific expression_system
3 Geobacillus stearothermophilus  (taxid:1422) Escherichia coli  (taxid:562)
expression_system_strain: BL21(DE3)pLysS
expression_system_vector_type: Plasmid
expression_system_plasmid: pET-30A(+)
other_details: THIS PROTEIN WAS ISOLATED FROM AN AS YET UNNAMED NOVEL STRAIN OF BACILLUS STEAROTHERMOPHILUS (SEE REF 2).
Authors : Johnson, S.J., Taylor, J.S., Beese, L.S.
Keywords : DNA POLYMERASE I, DNA REPLICATION, KLENOW FRAGMENT, PROTEIN-DNA-DNTP COMPLEX, CLOSED CONFORMATION, TRANSFERASE-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 1.95 Å )
Citation :

Processive DNA synthesis observed in a polymerase crystal suggests a mechanism for the prevention of frameshift mutations

Johnson, S.J.,Taylor, J.S.,Beese, L.S.
(2003)  Proc.Natl.Acad.Sci.USA  100 : 3895 - 3900

PubMed: 12649320
DOI: 10.1073/pnas.0630532100

Visualizing DNA Replication in a Catalytically Active Bacillus DNA Polymerase Crystal

Kiefer, J.R.,Mao, C.,Braman, J.C.  et al.
(1998)  Nature  391 : 304 - 307

DOI: 10.1038/34693

Crystal Structure of a Thermostable Bacillus DNA Polymerase I Large Fragment at 2.1 A Resolution

Kiefer, J.R.,Mao, C.,Hansen, C.J.  et al.
(1997)  Structure  5 : 95 - 108

DOI: 10.1016/S0969-2126(97)00169-X

Reaction

Chain : A, B
UniProt : P52026 (DPO1_GEOSE)
Reaction: EC: Evidence:
Physiological Direction:
a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 2.7.7.7 -
-