Brand  (β version)

PDB ID: 1LCO

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Ligands
Code Name Style Show Link
FMN Flavin mononucleotide PoSSuM
HEM Protoporphyrin ix containing Fe PoSSuM
PPY 3-phenylpyruvic acid PoSSuM
Non-standard Residues
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Structure summary

Code : 1LCO   PDBj   RCSB PDB   PDBe
Header : ELECTRON TRANSPORT
Title : X-RAY STRUCTURE OF TWO COMPLEXES OF THE Y143F FLAVOCYTOCHROME B2 MUTANT CRYSTALLIZED IN THE PRESENCE OF LACTATE OR PHENYL-LACTATE
Release Data : 1995-09-15
Compound :
mol_id molecule chains synonym
1 L-LACTATE DEHYDROGENASE A,B CYTOCHROME C OXIDOREDUCTASE, FLAVOCYTOCHROME B=2=
ec: 1.1.2.3
mutation: TYR 143 PHE
Source :
mol_id organism_scientific organism_common expression_system
1 Saccharomyces cerevisiae  (taxid:4932) Baker's yeast Escherichia coli  (taxid:562)
gene: PGR
expression_system_strain: MM294
expression_system_plasmid: PGR 401
expression_system_gene: PGR
Authors : Tegoni, M., Cambillau, C.
Keywords : ELECTRON TRANSPORT
Exp. method : X-RAY DIFFRACTION ( 2.9 Å )
Citation :

X-ray structure of two complexes of the Y143F flavocytochrome b2 mutant crystallized in the presence of lactate or phenyl lactate.

Tegoni, M.,Begotti, S.,Cambillau, C.
(1995)  Biochemistry  34 : 9840 - 9850

PubMed: 7632684
DOI: 10.1021/bi00031a004

The 2.6-Angstroms Refined Structure of the Escherichia Coli Recombinant Saccharomyces Cerevisiae Flavocytochrome B2-Sulfite Complex

Tegoni, M.,Cambillau, C.
(1994)  Protein Sci.  3 : 303

Structural Studies on Recombinant and Point Mutants of Flavocytochrome B2

Tegoni, M.,Cambillau, C.
(1994)  Biochimie  76 : 501

A Hypothetical Complex between Crystalline Flavocytochrome B2 and Cytochrome C

Tegoni, M.,White, S.A.,Roussel, A.  et al.
(1993)  Proteins  16 : 408

Tyr-143 Facilitates Interdomain Electron Transfer in Flavocytochrome B2

Miles, C.S.,Rouviere-Fourmy, N.,Lederer, F.  et al.
(1992)  Biochem.J.  285 : 187

High-Level Expression of Fully Active Yeast Cytochrome B2 in Escherichia Coli

Black, M.T.,White, S.A.,Reid, G.  et al.
(1989)  Biochem.J.  258 : 255

Reaction

Chain : A, B
UniProt : P00175 (CYB2_YEAST)
Reaction: EC: Evidence:
Physiological Direction:
(S)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + 2 H(+) + pyruvate 1.1.2.3 PubMed:11914072, PubMed:3004948, PubMed:4593578
left-to-right PubMed:3004948