Brand  (β version)

PDB ID: 1KNJ

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Ligands
Code Name Style Show Link
C5P Cytidine-5'-monophosphate PoSSuM
CDI 2c-methyl-D-erythritol 2,4-cyclodiphosphate PoSSuM
MN Manganese (II) ion PoSSuM
Non-standard Residues
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MSE Selenomethionine
Glycosylation
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Structure summary

Code : 1KNJ   PDBj   RCSB PDB   PDBe
Header : METAL BINDING PROTEIN
Title : Co-Crystal Structure of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate Synthase (ispF) from E. coli Involved in Mevalonate-Independent Isoprenoid Biosynthesis, Complexed with CMP/MECDP/Mn2+
Release Data : 2002-06-18
Compound :
mol_id molecule chains synonym
1 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase A MECPS
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli BL21(DE3)  (taxid:469008)
gene: ispF
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: PHIS8
Authors : Richard, S.B., Ferrer, J.L., Bowman, M.E., Lillo, A.M., Tetzlaff, C.N., Cane, D.E., Noel, J.P.
Keywords : isoprenoids, deoxyxylulose/methyl-erythritol-phosphate pathway, cyclodiphosphate, MEP, YgbB, ispF, MECDP, 2-C-methyl-D-erythritol-2, 4-cyclodiphosphate synthase, METAL BINDING PROTEIN
Exp. method : X-RAY DIFFRACTION ( 2.80 Å )
Citation :

Structure and mechanism of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase. An enzyme in the mevalonate-independent isoprenoid biosynthetic pathway.

Richard, S.B.,Ferrer, J.L.,Bowman, M.E.  et al.
(2002)  J.Biol.Chem.  277 : 8667 - 8672

PubMed: 11786530
DOI: 10.1074/jbc.C100739200

Structure of 4-diphosphocytidyl-2-C-methylerythritol Synthetase Involved in Mevalonate-Independent Isoprenoid Biosynthesis

Richard, S.B.,Bowman, M.E.,Kwiatkowski, W.  et al.
(2001)  Nat.Struct.Biol.  8 : 641 - 648

DOI: 10.1038/89691

Reaction

Chain : A
UniProt : P62617 (ISPF_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D- erythritol 2,4-cyclic diphosphate + CMP 4.6.1.12 HAMAP-Rule:MF_00107, PubMed:10694574, PubMed:22839733
-
4-CDP-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 3,4- cyclophosphate + CMP + H(+) - PubMed:10694574
-
Cofactor: Evidence: Note:
a divalent metal cation ECO:0000255 | HAMAP-Rule:MF_00107
ECO:0000269 | PubMed:10694574
ECO:0000269 | PubMed:11786530
ECO:0000269 | PubMed:11829504
ECO:0000269 | PubMed:11997478
ECO:0000269 | PubMed:15608374
ECO:0000269 | PubMed:16392111
ECO:0000269 | PubMed:16511114
ECO:0000269 | PubMed:19320487
Binds 1 divalent metal cation per subunit.